{"doi":"10.1073/pnas.2022403118","title":"Molecular assemblies of the catalytic domain of SOS with KRas and oncogenic mutants","abstract":"Significance Disrupting the interaction between Ras and SOS has emerged as an attractive therapeutic strategy. Here, we characterized the assembly of the catalytic domain of SOS (SOS cat ) with oncogenic KRas mutants using native mass spectrometry. By resolving distinct molecular species, we show KRas mutants engage SOS cat differently and the G13D KRas mutant robustly engages SOS cat . A structure of KRas G13D in complex with SOS cat shows repositioning of switch I and II regions within KRas G13D . Moreover, potent small-molecule Ras•SOS disruptors do not dissociate KRas G13D •SOS cat complexes when KRas G13D –GTP is bound at allosteric site of SOS cat . These results underscore the need for more potent Ras•SOS disruptors when considering targeting the disruption of different oncogenic Ras mutants in complex with SOS.","journal":"Proceedings of the National Academy of Sciences","year":2021,"id":165274,"datarank":0.5416376868966337,"base_score":3.6109179126442243,"endowment":3.6109179126442243,"self_citation_contribution":0.5416376868966337,"citation_network_contribution":0.0,"self_endowment_contribution":0.5416376868966337,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":36,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9501,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":501041,"name":"Mehdi Shirzadeh","orcid":"0000-0001-6306-9687","position":1,"is_corresponding":false},{"id":688264,"name":"Chang Liu","orcid":"0000-0001-9373-8458","position":2,"is_corresponding":false},{"id":688265,"name":"Samantha Schrecke","orcid":"0000-0002-5228-0310","position":3,"is_corresponding":false},{"id":688813,"name":"Charles Packianathan","orcid":null,"position":4,"is_corresponding":false},{"id":323307,"name":"David H. Russell","orcid":"0000-0003-0830-3914","position":5,"is_corresponding":false},{"id":342826,"name":"Minglei Zhao","orcid":"0000-0001-5832-6060","position":6,"is_corresponding":false},{"id":297720,"name":"Arthur Laganowsky","orcid":"0000-0001-5012-5547","position":7,"is_corresponding":false},{"id":688812,"name":"Zahra Moghadamchargari","orcid":null,"position":0,"is_corresponding":true}],"reference_count":52,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:45:36.425208Z","pmid":"33723061","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}