{"doi":"10.1073/pnas.2021943118","title":"A short ORF-encoded transcriptional regulator","abstract":"Recent technological advances have expanded the annotated protein coding content of mammalian genomes, as hundreds of previously unidentified, short open reading frame (ORF)-encoded peptides (SEPs) have now been found to be translated. Although several studies have identified important physiological roles for this emerging protein class, a general method to define their interactomes is lacking. Here, we demonstrate that genetic incorporation of the photo-crosslinking noncanonical amino acid AbK into SEP transgenes allows for the facile identification of SEP cellular interaction partners using affinity-based methods. From a survey of seven SEPs, we report the discovery of short ORF-encoded histone binding protein (SEHBP), a conserved microprotein that interacts with chromatin-associated proteins, localizes to discrete genomic loci, and induces a robust transcriptional program when overexpressed in human cells. This work affords a straightforward method to help define the physiological roles of SEPs and demonstrates its utility by identifying SEHBP as a short ORF-encoded transcription factor.","journal":"Proceedings of the National Academy of Sciences","year":2021,"id":154993,"datarank":0.6190701577567639,"base_score":4.127134385045092,"endowment":4.127134385045092,"self_citation_contribution":0.6190701577567639,"citation_network_contribution":0.0,"self_endowment_contribution":0.6190701577567639,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":61,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9523,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":657638,"name":"Insha Ahmad","orcid":null,"position":1,"is_corresponding":false},{"id":419935,"name":"Yeonjin Ko","orcid":"0009-0006-2511-0857","position":2,"is_corresponding":false},{"id":469466,"name":"Yuxiang Zhang","orcid":"0000-0002-5110-5684","position":3,"is_corresponding":false},{"id":17848,"name":"Thomas F. Martinez","orcid":"0000-0002-4011-8164","position":4,"is_corresponding":false},{"id":90672,"name":"Jolene K. Diedrich","orcid":"0000-0001-6489-4558","position":5,"is_corresponding":false},{"id":656576,"name":"Qian Chu","orcid":"0000-0001-6550-6975","position":6,"is_corresponding":false},{"id":298836,"name":"James J. Moresco","orcid":"0000-0003-1178-1642","position":7,"is_corresponding":false},{"id":116744,"name":"Michael A. Erb","orcid":"0000-0001-9993-3481","position":8,"is_corresponding":false},{"id":17868,"name":"Alan Saghatelian","orcid":"0000-0002-0427-563X","position":9,"is_corresponding":false},{"id":107931,"name":"Peter G. Schultz","orcid":"0000-0003-3188-1202","position":10,"is_corresponding":false},{"id":310926,"name":"Michael J. Bollong","orcid":"0000-0001-9439-1476","position":11,"is_corresponding":false},{"id":376105,"name":"Minseob Koh","orcid":"0000-0003-1935-4776","position":0,"is_corresponding":true}],"reference_count":35,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:43:59.154159Z","pmid":"33468658","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}