{"doi":"10.1073/pnas.2000111117","title":"Characterization of the coformycin biosynthetic gene cluster in <i>Streptomyces kaniharaensis</i>","abstract":"gene cluster responsible for coformycin biosynthesis is identified. Reconstitution of the coformycin biosynthetic pathway in vitro demonstrates that it overlaps significantly with the early stages of l-histidine biosynthesis. Committed entry into the coformycin pathway takes place via conversion of a shared branch point intermediate to 8-ketocoformycin-[Formula: see text]-monophosphate catalyzed by CofB, which is a homolog of succinylaminoimidazolecarboxamide ribotide (SAICAR) synthetase. This reaction appears to proceed via a Dieckmann cyclization and a retro-aldol elimination, releasing ammonia and D-erythronate-4-phosphate as coproducts. Completion of coformycin biosynthesis involves reduction and dephosphorylation of the CofB product, with the former reaction being catalyzed by the NADPH-dependent dehydrogenase CofA. CofB also shows activation by adenosine triphosphate (ATP) despite the reaction requiring neither a phosphorylated nor an adenylated intermediate. This may serve to help regulate metabolic partitioning between the l-histidine and coformycin pathways.","journal":"Proceedings of the National Academy of Sciences","year":2020,"id":81109,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9444,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":421160,"name":"Mark W. Ruszczycky","orcid":null,"position":1,"is_corresponding":false},{"id":419935,"name":"Yeonjin Ko","orcid":"0009-0006-2511-0857","position":2,"is_corresponding":false},{"id":419936,"name":"Shao‐An Wang","orcid":"0000-0002-5473-4195","position":3,"is_corresponding":false},{"id":419937,"name":"Yasushi Ogasawara","orcid":"0000-0002-3144-3985","position":4,"is_corresponding":false},{"id":419938,"name":"Minje Kim","orcid":"0000-0003-3513-8328","position":5,"is_corresponding":false},{"id":419939,"name":"Hung‐wen Liu","orcid":"0000-0001-8953-4794","position":6,"is_corresponding":false},{"id":419934,"name":"Daan Ren","orcid":"0000-0002-1309-5382","position":0,"is_corresponding":true}],"reference_count":40,"raw_metadata":null,"created_at":"2026-07-18T21:52:34.401799Z","pmid":"32350138","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}