{"doi":"10.1073/pnas.1919535117","title":"Building blocks are synthesized on demand during the yeast cell cycle","abstract":"<jats:p>\n                    For cells to replicate, a sufficient supply of biosynthetic precursors is needed, necessitating the concerted action of metabolism and protein synthesis during progressive phases of cell division. A global understanding of which biosynthetic processes are involved and how they are temporally regulated during replication is, however, currently lacking. Here, quantitative multiomics analysis is used to generate a holistic view of the eukaryal cell cycle, using the budding yeast\n                    <jats:italic>Saccharomyces cerevisiae</jats:italic>\n                    . Protein synthesis and central carbon pathways such as glycolysis and amino acid metabolism are shown to synchronize their respective abundance profiles with division, with pathway-specific changes in metabolite abundance also being reflected by a relative increase in mitochondrial volume, as shown by quantitative fluorescence microscopy. These results show biosynthetic precursor production to be temporally regulated to meet phase-specific demands of eukaryal cell division.\n                  </jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2020,"id":643747,"datarank":0.6064576901751826,"base_score":4.04305126783455,"endowment":4.04305126783455,"self_citation_contribution":0.6064576901751826,"citation_network_contribution":0.0,"self_endowment_contribution":0.6064576901751826,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":56,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":99110,"name":"Jakub Orzechowski Westholm","orcid":"0000-0002-6849-6220","position":1,"is_corresponding":false},{"id":1675138,"name":"Sergo Kasvandik","orcid":"0000-0003-0218-2410","position":2,"is_corresponding":false},{"id":1675139,"name":"Francesca Di Bartolomeo","orcid":"0000-0002-4302-0701","position":3,"is_corresponding":false},{"id":1675140,"name":"Maurizio Mormino","orcid":"0000-0003-2055-5081","position":4,"is_corresponding":false},{"id":110749,"name":"Jens Nielsen","orcid":"0000-0002-9955-6003","position":5,"is_corresponding":false},{"id":1675137,"name":"Kate Campbell","orcid":"0000-0002-4173-5260","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Building blocks are synthesized on demand during the yeast cell cycle","abstract":"<jats:p>\n                    For cells to replicate, a sufficient supply of biosynthetic precursors is needed, necessitating the concerted action of metabolism and protein synthesis during progressive phases of cell division. A global understanding of which biosynthetic processes are involved and how they are temporally regulated during replication is, however, currently lacking. Here, quantitative multiomics analysis is used to generate a holistic view of the eukaryal cell cycle, using the budding yeast\n                    <jats:italic>Saccharomyces cerevisiae</jats:italic>\n                    . Protein synthesis and central carbon pathways such as glycolysis and amino acid metabolism are shown to synchronize their respective abundance profiles with division, with pathway-specific changes in metabolite abundance also being reflected by a relative increase in mitochondrial volume, as shown by quantitative fluorescence microscopy. These results show biosynthetic precursor production to be temporally regulated to meet phase-specific demands of eukaryal cell division.\n                  </jats:p>","is_dataset_classified":null,"base_score":4.04305126783455,"endowment":4.04305126783455,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"32213592","pmcid":"PMC7149230","openalex_id":"https://openalex.org/W3014047879","authors":[],"funders":[{"funder_name":"Novo Nordisk","grant_id":"NNF10CC1016517","title":null},{"funder_name":"Knut och Alice Wallenbergs Stiftelse","grant_id":"2015-0279","title":null},{"funder_name":"NNF Center for Biosustainability","grant_id":"Yeast Cell Factories","title":null},{"funder_name":"Novo Nordisk Fonden","grant_id":"NNF17SA0032514-4","title":null}],"total_grants":4,"fwci":2.3115,"citation_percentile":0.89308689,"influential_citations":0,"citation_trend":[{"year":2020,"count":6},{"year":2021,"count":6},{"year":2022,"count":9},{"year":2023,"count":10},{"year":2024,"count":7},{"year":2025,"count":14},{"year":2026,"count":4}],"oa_status":"bronze","license":"https://www.pnas.org/site/aboutpnas/licenses.xhtml","oa_locations":[{"url":"https://www.pnas.org/content/pnas/117/14/7575.full.pdf","host_type":"journal"},{"url":"https://www.pnas.org/content/pnas/117/14/7575.full.pdf","host_type":"publisher"},{"url":"http://www.pnas.org/syndication/doi/10.1073/pnas.1919535117","host_type":"publisher"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.1919535117","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.1919535117","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/32213592","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7149230","host_type":"repository"},{"url":"https://orbit.dtu.dk/en/publications/1d241dba-41ea-48ca-9853-4692f469fa17","host_type":"repository"},{"url":"https://research.chalmers.se/en/publication/516595","host_type":"repository"},{"url":"https://research.chalmers.se/en/publication/024130ca-9db4-4bc0-aabe-adc6efbe771b","host_type":"repository"}],"fields_of_study":["Fungal and yeast genetics research","Microbial Metabolic Engineering and Bioproduction","Bioinformatics and Genomic Networks","Carbon","Cell Cycle","Genomics","Protein Biosynthesis","Saccharomyces cerevisiae"],"mesh_terms":["Carbon","Cell Cycle","Saccharomyces cerevisiae","Protein Biosynthesis","Genomics"],"keywords":["Saccharomyces cerevisiae","Yeast","Cell cycle","Cell division","Biochemistry","Biology","Metabolite","Metabolism","Metabolic pathway","Citric acid cycle","Cell","Cell biology","Glycolysis","Protein biosynthesis","Budding yeast","Absolute Quantitation","Multiomics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"arrayexpress"},{"name":"pxd"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-08T18:38:55.819845Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}