{"doi":"10.1073/pnas.1913448117","title":"Coordination of mRNA and tRNA methylations by TRMT10A","abstract":"<jats:p>\n                    The posttranscriptional modification of messenger RNA (mRNA) and transfer RNA (tRNA) provides an additional layer of regulatory complexity during gene expression. Here, we show that a tRNA methyltransferase, TRMT10A, interacts with an mRNA demethylase FTO (ALKBH9), both in vitro and inside cells. TRMT10A installs\n                    <jats:italic>N</jats:italic>\n                    <jats:sup>1</jats:sup>\n                    -methylguanosine (m\n                    <jats:sup>1</jats:sup>\n                    G) in tRNA, and FTO performs demethylation on\n                    <jats:italic>N</jats:italic>\n                    <jats:sup>6</jats:sup>\n                    -methyladenosine (m\n                    <jats:sup>6</jats:sup>\n                    A) and\n                    <jats:italic>N</jats:italic>\n                    <jats:sup>6</jats:sup>\n                    ,2′-\n                    <jats:italic>O</jats:italic>\n                    -dimethyladenosine (m\n                    <jats:sup>6</jats:sup>\n                    A\n                    <jats:sub>m</jats:sub>\n                    ) in mRNA. We show that TRMT10A ablation not only leads to decreased m\n                    <jats:sup>1</jats:sup>\n                    G in tRNA but also significantly increases m\n                    <jats:sup>6</jats:sup>\n                    A levels in mRNA. Cross-linking and immunoprecipitation, followed by high-throughput sequencing results show that TRMT10A shares a significant overlap of associated mRNAs with FTO, and these mRNAs have accelerated decay rates potentially through the regulation by a specific m\n                    <jats:sup>6</jats:sup>\n                    A reader, YTHDF2. Furthermore, transcripts with increased m\n                    <jats:sup>6</jats:sup>\n                    A upon TRMT10A ablation contain an overrepresentation of m\n                    <jats:sup>1</jats:sup>\n                    G9-containing tRNAs codons read by tRNA\n                    <jats:sup>Gln(TTG)</jats:sup>\n                    , tRNA\n                    <jats:sup>Arg(CCG)</jats:sup>\n                    , and tRNA\n                    <jats:sup>Thr(CGT)</jats:sup>\n                    . These findings collectively reveal the presence of coordinated mRNA and tRNA methylations and demonstrate a mechanism for regulating gene expression through the interactions between mRNA and tRNA modifying enzymes.\n                  </jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2020,"id":609294,"datarank":0.6284482113039639,"base_score":4.189654742026425,"endowment":4.189654742026425,"self_citation_contribution":0.6284482113039639,"citation_network_contribution":0.0,"self_endowment_contribution":0.6284482113039639,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":65,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":548291,"name":"Hui Shen","orcid":"0000-0002-7456-5536","position":1,"is_corresponding":false},{"id":758840,"name":"Julian Stoute","orcid":"0000-0003-4735-4706","position":2,"is_corresponding":false},{"id":1001710,"name":"Amber Yanas","orcid":"0000-0001-7817-6987","position":3,"is_corresponding":false},{"id":509633,"name":"Yixiao Cui","orcid":"0000-0001-7337-3674","position":4,"is_corresponding":false},{"id":135233,"name":"Yuyu Zhang","orcid":null,"position":5,"is_corresponding":false},{"id":297703,"name":"Kathy Fange Liu","orcid":"0000-0001-5943-3888","position":6,"is_corresponding":false},{"id":1565980,"name":"R. Jordan Ontiveros","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Coordination of mRNA and tRNA methylations by TRMT10A","abstract":"<jats:p>\n                    The posttranscriptional modification of messenger RNA (mRNA) and transfer RNA (tRNA) provides an additional layer of regulatory complexity during gene expression. Here, we show that a tRNA methyltransferase, TRMT10A, interacts with an mRNA demethylase FTO (ALKBH9), both in vitro and inside cells. TRMT10A installs\n                    <jats:italic>N</jats:italic>\n                    <jats:sup>1</jats:sup>\n                    -methylguanosine (m\n                    <jats:sup>1</jats:sup>\n                    G) in tRNA, and FTO performs demethylation on\n                    <jats:italic>N</jats:italic>\n                    <jats:sup>6</jats:sup>\n                    -methyladenosine (m\n                    <jats:sup>6</jats:sup>\n                    A) and\n                    <jats:italic>N</jats:italic>\n                    <jats:sup>6</jats:sup>\n                    ,2′-\n                    <jats:italic>O</jats:italic>\n                    -dimethyladenosine (m\n                    <jats:sup>6</jats:sup>\n                    A\n                    <jats:sub>m</jats:sub>\n                    ) in mRNA. We show that TRMT10A ablation not only leads to decreased m\n                    <jats:sup>1</jats:sup>\n                    G in tRNA but also significantly increases m\n                    <jats:sup>6</jats:sup>\n                    A levels in mRNA. Cross-linking and immunoprecipitation, followed by high-throughput sequencing results show that TRMT10A shares a significant overlap of associated mRNAs with FTO, and these mRNAs have accelerated decay rates potentially through the regulation by a specific m\n                    <jats:sup>6</jats:sup>\n                    A reader, YTHDF2. Furthermore, transcripts with increased m\n                    <jats:sup>6</jats:sup>\n                    A upon TRMT10A ablation contain an overrepresentation of m\n                    <jats:sup>1</jats:sup>\n                    G9-containing tRNAs codons read by tRNA\n                    <jats:sup>Gln(TTG)</jats:sup>\n                    , tRNA\n                    <jats:sup>Arg(CCG)</jats:sup>\n                    , and tRNA\n                    <jats:sup>Thr(CGT)</jats:sup>\n                    . These findings collectively reveal the presence of coordinated mRNA and tRNA methylations and demonstrate a mechanism for regulating gene expression through the interactions between mRNA and tRNA modifying enzymes.\n                  </jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"32213595","pmcid":null,"openalex_id":"https://openalex.org/W32213595","authors":[],"funders":[{"funder_name":"HHS | NIH | National Institute of General Medical Sciences","grant_id":"1R35GM133721-01","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R35 GM133721","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM132039","title":null}],"total_grants":3,"fwci":0.0,"citation_percentile":0.00940014,"influential_citations":0,"citation_trend":[],"oa_status":"bronze","license":"https://www.pnas.org/site/aboutpnas/licenses.xhtml","oa_locations":[{"url":"https://www.pnas.org/content/pnas/117/14/7782.full.pdf","host_type":"publisher"},{"url":"http://www.pnas.org/syndication/doi/10.1073/pnas.1913448117","host_type":"publisher"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.1913448117","host_type":"publisher"},{"url":"https://dialnet.unirioja.es/descarga/articulo/2900094.pdf","host_type":"journal"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7149399","host_type":"repository"}],"fields_of_study":["Classical Antiquity Studies","Adenosine","Alpha-Ketoglutarate-Dependent Dioxygenase FTO","Gene Expression Regulation","HEK293 Cells","High-Throughput Nucleotide Sequencing","Humans","Methylation","Methyltransferases","RNA, Messenger","RNA, Transfer","RNA-Binding Proteins","tRNA Methyltransferases"],"mesh_terms":["Adenosine","Alpha-Ketoglutarate-Dependent Dioxygenase FTO","Gene Expression Regulation","HEK293 Cells","High-Throughput Nucleotide Sequencing","Humans","Methylation","Methyltransferases","RNA, Messenger","RNA, Transfer","RNA-Binding Proteins","tRNA Methyltransferases"],"keywords":["Computer science","Art","Political science","mRNA","modification","tRNA"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-31T05:41:56.811642Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}