{"doi":"10.1073/pnas.1818680116","title":"Dynamics of DNA replication in a eukaryotic cell","abstract":"<jats:title>Significance</jats:title>\n                  <jats:p>\n                    All eukaryotic cells initiate DNA replication at multiple genomic sites. For most cell types these sites lack a well-defined sequence signature, so it is not understood how they are selected. To analyze the factors that influence initiation site selection and determine the dynamics of replication throughout the genome, we developed an integrative computational model of DNA replication in the model organism\n                    <jats:italic>Schizosaccharomyces pombe</jats:italic>\n                    . This analysis showed that the locations of initiation sites are determined not only by the sequence preferences of the\n                    <jats:italic>S. pombe</jats:italic>\n                    origin recognition complex (ORC), but also by the interference of transcription with the formation of prereplication complexes. Our findings suggest that transcription has influenced evolution of the binding properties of ORC and replication dynamics in eukaryotes.\n                  </jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2019,"id":597654,"datarank":0.5897738449086489,"base_score":3.9318256327243257,"endowment":3.9318256327243257,"self_citation_contribution":0.5897738449086489,"citation_network_contribution":0.0,"self_endowment_contribution":0.5897738449086489,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":50,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1531168,"name":"A. John Callegari","orcid":"0000-0003-3959-1947","position":1,"is_corresponding":false},{"id":1285152,"name":"Thomas Kelly","orcid":"0000-0001-6285-1925","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Dynamics of DNA replication in a eukaryotic cell","abstract":"<jats:title>Significance</jats:title>\n                  <jats:p>\n                    All eukaryotic cells initiate DNA replication at multiple genomic sites. For most cell types these sites lack a well-defined sequence signature, so it is not understood how they are selected. To analyze the factors that influence initiation site selection and determine the dynamics of replication throughout the genome, we developed an integrative computational model of DNA replication in the model organism\n                    <jats:italic>Schizosaccharomyces pombe</jats:italic>\n                    . This analysis showed that the locations of initiation sites are determined not only by the sequence preferences of the\n                    <jats:italic>S. pombe</jats:italic>\n                    origin recognition complex (ORC), but also by the interference of transcription with the formation of prereplication complexes. Our findings suggest that transcription has influenced evolution of the binding properties of ORC and replication dynamics in eukaryotes.\n                  </jats:p>","is_dataset_classified":null,"base_score":3.9318256327243257,"endowment":3.9318256327243257,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30718387","pmcid":"PMC6421431","openalex_id":"https://openalex.org/W2912756262","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"P30 CA008748","title":null}],"total_grants":1,"fwci":2.9582,"citation_percentile":0.92269824,"influential_citations":0,"citation_trend":[{"year":2019,"count":5},{"year":2020,"count":14},{"year":2021,"count":13},{"year":2022,"count":7},{"year":2023,"count":4},{"year":2024,"count":4},{"year":2025,"count":3}],"oa_status":"bronze","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","oa_locations":[{"url":"https://www.pnas.org/content/pnas/116/11/4973.full.pdf","host_type":"journal"},{"url":"https://www.pnas.org/content/pnas/116/11/4973.full.pdf","host_type":"publisher"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.1818680116","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.1818680116","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/30718387","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6421431","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC6421431","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC6421431?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["DNA Repair Mechanisms","Genomics and Chromatin Dynamics","Microtubule and mitosis dynamics","AT Rich Sequence","Chromosomes, Fungal","Computer Simulation","DNA Replication","DNA Replication Timing","DNA-Directed RNA Polymerases","Eukaryotic Cells","Genome, Fungal","Models, Biological","Origin Recognition Complex","Probability","Schizosaccharomyces","Schizosaccharomyces pombe Proteins","Transcription, Genetic"],"mesh_terms":["Computer Simulation","DNA Replication","Eukaryotic Cells","Models, Biological","Probability","DNA-Directed RNA Polymerases","Schizosaccharomyces","Transcription, Genetic","Chromosomes, Fungal","Genome, Fungal","AT Rich Sequence","Schizosaccharomyces pombe Proteins","DNA Replication Timing","Origin Recognition Complex"],"keywords":["Schizosaccharomyces pombe","Origin recognition complex","Biology","Pre-replication complex","Origin of replication","DNA replication","Licensing factor","Eukaryotic DNA replication","Schizosaccharomyces","Genetics","Control of chromosome duplication","Replication timing","Minichromosome maintenance","Computational biology","Genome","Ter protein","DNA","Saccharomyces cerevisiae","Gene","Transcriptional Interference","Replication Origins","Replication Dynamics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T13:50:59.478353Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}