{"doi":"10.1073/pnas.1810647115","title":"SIR proteins create compact heterochromatin fibers","abstract":"<jats:title>Significance</jats:title>\n                  <jats:p>Eukaryotic chromosomes are organized into specialized, nucleoprotein domains that can regulate nuclear functions. Heterochromatin is a silenced chromatin region essential for maintaining genomic stability and driving developmental processes. Cell biological studies have indicated that heterochromatin is highly condensed, though the complicated structure and dynamics of heterochromatin have rendered it difficult to characterize. Here we reconstitute heterochromatin fibers with purified components and exploit a powerful combination of sedimentation velocity, molecular modeling, and atomic force microscopy to characterize the stoichiometry and condensed conformation of heterochromatin fibers.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2018,"id":624846,"datarank":0.47670807455219194,"base_score":3.1780538303479458,"endowment":3.1780538303479458,"self_citation_contribution":0.47670807455219194,"citation_network_contribution":0.0,"self_endowment_contribution":0.47670807455219194,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":23,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":356680,"name":"Subhadip Senapati","orcid":"0000-0001-9071-1911","position":1,"is_corresponding":false},{"id":1615414,"name":"Mehmet F. Bolukbasi","orcid":null,"position":2,"is_corresponding":false},{"id":120623,"name":"Scot A. Wolfe","orcid":null,"position":3,"is_corresponding":false},{"id":347459,"name":"Stuart Lindsay","orcid":"0000-0002-9364-6542","position":4,"is_corresponding":false},{"id":68359,"name":"Craig L. Peterson","orcid":"0000-0002-9448-555X","position":5,"is_corresponding":false},{"id":115013,"name":"Sarah G. Swygert","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"SIR proteins create compact heterochromatin fibers","abstract":"<jats:title>Significance</jats:title>\n                  <jats:p>Eukaryotic chromosomes are organized into specialized, nucleoprotein domains that can regulate nuclear functions. Heterochromatin is a silenced chromatin region essential for maintaining genomic stability and driving developmental processes. Cell biological studies have indicated that heterochromatin is highly condensed, though the complicated structure and dynamics of heterochromatin have rendered it difficult to characterize. Here we reconstitute heterochromatin fibers with purified components and exploit a powerful combination of sedimentation velocity, molecular modeling, and atomic force microscopy to characterize the stoichiometry and condensed conformation of heterochromatin fibers.</jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30455303","pmcid":"PMC6298083","openalex_id":null,"authors":[],"funders":[{"funder_name":"HHS | NIH | National Institute of General Medical Sciences","grant_id":"GM54096","title":null},{"funder_name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","grant_id":"R01AI117839","title":null},{"funder_name":"HHS | NIH | National Cancer Institute","grant_id":"U54 CA143862","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM054096","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R35 GM122519","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R01AI117839-04","title":"Next generation hybrid nucleases for precise excision of latent HIV-1 provirus"},{"funder_name":"National Institutes of Health","grant_id":"5U54CA143862-04","title":"A Center for the Convergence of Physical Science and Cancer Biology"}],"total_grants":7,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"bronze","license":"http://www.pnas.org/site/aboutpnas/licenses.xhtml","oa_locations":[{"url":"https://www.pnas.org/content/pnas/115/49/12447.full.pdf","host_type":"publisher"},{"url":"http://www.pnas.org/syndication/doi/10.1073/pnas.1810647115","host_type":"publisher"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.1810647115","host_type":"publisher"},{"url":"https://doi.org/10.1101/346296","host_type":""},{"url":"https://www.biorxiv.org/content/biorxiv/early/2018/06/13/346296.full.pdf","host_type":""},{"url":"https://doi.org/10.1073/pnas.1810647115","host_type":""},{"url":"https://europepmc.org/articles/pmc6298083?pdf=render","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/30455303","host_type":""},{"url":"https://dx.doi.org/10.1101/346296","host_type":""},{"url":"https://dx.doi.org/10.1073/pnas.1810647115","host_type":""},{"url":"http://dx.doi.org/10.1101/346296","host_type":""}],"fields_of_study":["0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Heterochromatin","Saccharomyces cerevisiae","Silent Information Regulator Proteins, Saccharomyces cerevisiae","Histones","Gene Expression Regulation, Fungal","Protein Binding"],"keywords":["SIR","Sedimentation","Heterochromatin","chromatin","Histones","Gene Expression Regulation, Fungal","Saccharomyces cerevisiae","Silent Information Regulator Proteins, Saccharomyces cerevisiae","Protein Binding"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T05:02:30.786534Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}