{"doi":"10.1073/pnas.1722281115","title":"Capping protein regulates actin dynamics during cytokinetic midbody maturation","abstract":"<jats:title>Significance</jats:title>\n                  <jats:p>Actin dynamics drive many steps of cell division. Here, we show that the actin capping protein (CP) is unexpectedly involved in midbody maturation, a poorly understood phase of the cell cycle where cells remodel their intercellular bridges to prepare for separation. The loss of CP results in excessive filamentous actin throughout the cell cycle, but only postfurrowing cytokinesis is inhibited. We propose that optimal actin filament function is achieved by a balance between CP-dependent filament capping and formin-driven polymerization. This raises the intriguing possibility that cells utilize specific types of actin filament networks to progress through division. This finding has profound implications on understanding actin-dependent processes such as cell division, migration, adhesion, and morphogenesis.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2018,"id":588591,"datarank":1.5489861111592362,"base_score":3.9318256327243257,"endowment":3.9318256327243257,"self_citation_contribution":0.5897738449086489,"citation_network_contribution":0.9592122662505873,"self_endowment_contribution":0.5897738449086489,"citer_contribution":0.9592122662505873,"corpus_percentile":null,"corpus_rank":null,"citation_count":50,"citer_count":43,"citers_with_citation_signal":33,"citers_with_endowment":33,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1505821,"name":"Federico Donà","orcid":null,"position":1,"is_corresponding":false},{"id":1505822,"name":"Paul Osenberg","orcid":null,"position":2,"is_corresponding":false},{"id":1505823,"name":"Jeremy G. Carlton","orcid":"0000-0002-7255-1894","position":3,"is_corresponding":false},{"id":1505824,"name":"Ulrike S. Eggert","orcid":null,"position":4,"is_corresponding":false},{"id":1505820,"name":"Stephen J. Terry","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Capping protein regulates actin dynamics during cytokinetic midbody maturation","abstract":"During cytokinesis, a cleavage furrow generated by actomyosin ring contraction is restructured into the midbody, a platform for the assembly of the abscission machinery that controls the final separation of daughter cells. The polymerization state of F-actin is important during assembly, ingression, disassembly, and closure of the contractile ring and for the cytoskeletal remodeling that accompanies midbody formation and progression to abscission. Actin filaments must be cleared from the abscission sites before the final cut can take place. Although many conserved proteins interact with and influence the polymerization state of actin filaments, it is poorly understood how they regulate cytokinesis in higher eukaryotes. We report here that the actin capping protein (CP), a barbed end actin binding protein, participates in the control of actin polymerization during later stages of cytokinesis in human cells. Cells depleted of CP furrow and form early midbodies, but they fail cytokinesis. Appropriate recruitment of the ESCRT-III abscission machinery to the midbody is impaired, preventing the cell from progressing to the abscission stage. To generate actin filaments of optimal length, different actin nucleators, such as formins, balance CP's activity. Loss of actin capping activity leads to excessive accumulation of formin-based linear actin filaments. Depletion of the formin FHOD1 results in partial rescue of CP-induced cytokinesis failure, suggesting that it can antagonize CP activity during midbody maturation. Our work suggests that the actin cytoskeleton is remodeled in a stepwise manner during cytokinesis, with different regulators at different stages required for successful progression to abscission.","is_dataset_classified":null,"base_score":3.9318256327243257,"endowment":3.9318256327243257,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29439200","pmcid":"PMC5834733","openalex_id":"https://openalex.org/W2791020137","authors":[],"funders":[{"funder_name":"European Research Council","grant_id":"306659","title":null},{"funder_name":"Wellcome Trust","grant_id":"093603/Z/10/Z","title":null},{"funder_name":"Wellcome Trust","grant_id":"110060/Z/15/Z","title":null},{"funder_name":"European Research Council","grant_id":"206346","title":null},{"funder_name":"Wellcome Trust","grant_id":"206346/Z/17/Z","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM082834","title":null},{"funder_name":"Wellcome Trust","grant_id":"","title":null},{"funder_name":"Wellcome Trust","grant_id":"","title":null}],"total_grants":8,"fwci":2.879,"citation_percentile":0.9073226,"influential_citations":0,"citation_trend":[{"year":2018,"count":5},{"year":2019,"count":8},{"year":2020,"count":7},{"year":2021,"count":5},{"year":2022,"count":9},{"year":2023,"count":5},{"year":2024,"count":5},{"year":2025,"count":3},{"year":2026,"count":3}],"oa_status":"bronze","license":"other-oa","oa_locations":[{"url":"https://www.pnas.org/content/pnas/115/9/2138.full.pdf","host_type":"journal"},{"url":"https://www.pnas.org/content/pnas/115/9/2138.full.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.1722281115","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/29439200","host_type":"repository"},{"url":"https://kclpure.kcl.ac.uk/portal/en/publications/d03d78fe-4562-4a70-9508-68d80073eb73","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5834733","host_type":"repository"},{"url":"https://kclpure.kcl.ac.uk/ws/files/109743569/2138.full.pdf","host_type":"repository"}],"fields_of_study":["Cellular Mechanics and Interactions","Cell Adhesion Molecules Research","RNA Research and Splicing","Actin Capping Proteins","Actin Cytoskeleton","Actins","Cell Membrane","Cytokinesis","Endosomal Sorting Complexes Required for Transport","Epithelial Cells","Epithelium, Corneal","Fetal Proteins","Formins","Gene Expression Regulation","HeLa Cells","Humans","Microfilament Proteins","Nuclear Proteins"],"mesh_terms":["Formins","Actins","Cell Membrane","Epithelial Cells","Fetal Proteins","Gene Expression Regulation","HeLa Cells","Humans","Microfilament Proteins","Actin Cytoskeleton","Nuclear Proteins","Epithelium, Corneal","Cytokinesis","Actin Capping Proteins","Endosomal Sorting Complexes Required for Transport","Hela Cells"],"keywords":["Cell biology","Midbody","Cytokinesis","Actin","Dynamics (music)","Biology","Chemistry","Biophysics","Cell division","Cell","Physics","Biochemistry","F-actin","Capping Protein","Formins"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-22T01:25:30.651692Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}