{"doi":"10.1073/pnas.1519730113","title":"Positive-strand RNA viruses stimulate host phosphatidylcholine synthesis at viral replication sites","abstract":"<jats:title>Significance</jats:title>\n                  <jats:p>Positive-strand RNA viruses [(+)RNA viruses] include many important human, animal, and plant pathogens. A highly conserved and indispensable feature of (+)RNA virus infection is that these viruses proliferate and reorganize host membranes to assemble viral replication complexes (VRCs). We show that brome mosaic virus (BMV) stimulates phosphatidylcholine (PC) synthesis at the viral replication sites. BMV recruits a host enzyme involved in PC synthesis to support proper VRC formation and genomic replication. We further show that hepatitis C virus and poliovirus also promote accumulation of PC at the viral replication sites, revealing a feature common to a group of (+)RNA viruses. This virus-specific step can be targeted to develop a broad-spectrum antiviral strategy with the least side effects on host growth.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2016,"id":596727,"datarank":0.7023196840686331,"base_score":4.68213122712422,"endowment":4.68213122712422,"self_citation_contribution":0.7023196840686331,"citation_network_contribution":0.0,"self_endowment_contribution":0.7023196840686331,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":107,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1528382,"name":"Zhenlu Zhang","orcid":null,"position":1,"is_corresponding":false},{"id":1528383,"name":"Vineela Chukkapalli","orcid":null,"position":2,"is_corresponding":false},{"id":1528384,"name":"Jules A. Nchoutmboube","orcid":null,"position":3,"is_corresponding":false},{"id":733602,"name":"Jianhui Li","orcid":"0009-0000-5005-8508","position":4,"is_corresponding":false},{"id":536228,"name":"Glenn Randall","orcid":"0000-0001-7417-3615","position":5,"is_corresponding":false},{"id":458481,"name":"George A. Belov","orcid":"0000-0002-0892-1731","position":6,"is_corresponding":false},{"id":316625,"name":"Xiaofeng Wang","orcid":"0000-0001-8212-6931","position":7,"is_corresponding":false},{"id":765414,"name":"Jiantao Zhang","orcid":"0000-0002-2875-9743","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Positive-strand RNA viruses stimulate host phosphatidylcholine synthesis at viral replication sites","abstract":"<jats:title>Significance</jats:title>\n                  <jats:p>Positive-strand RNA viruses [(+)RNA viruses] include many important human, animal, and plant pathogens. A highly conserved and indispensable feature of (+)RNA virus infection is that these viruses proliferate and reorganize host membranes to assemble viral replication complexes (VRCs). We show that brome mosaic virus (BMV) stimulates phosphatidylcholine (PC) synthesis at the viral replication sites. BMV recruits a host enzyme involved in PC synthesis to support proper VRC formation and genomic replication. We further show that hepatitis C virus and poliovirus also promote accumulation of PC at the viral replication sites, revealing a feature common to a group of (+)RNA viruses. This virus-specific step can be targeted to develop a broad-spectrum antiviral strategy with the least side effects on host growth.</jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26858414","pmcid":"PMC4776486","openalex_id":null,"authors":[],"funders":[{"funder_name":"NSF | BIO | Division of Integrative Organismal Systems","grant_id":"1265260","title":null},{"funder_name":"HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases","grant_id":"DK102883","title":null},{"funder_name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","grant_id":"AI115383","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI125561","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R21 AI115383","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"R01 DK102883","title":null}],"total_grants":6,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"bronze","license":"http://www.pnas.org/preview_site/misc/userlicense.xhtml","oa_locations":[{"url":"https://www.pnas.org/content/pnas/113/8/E1064.full.pdf","host_type":"publisher"},{"url":"http://www.pnas.org/syndication/doi/10.1073/pnas.1519730113","host_type":"publisher"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.1519730113","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":["Intracellular Membranes","Endoplasmic Reticulum","Bromovirus","Saccharomyces cerevisiae","Hordeum","Phosphatidylcholines","Virus Replication","Plant Diseases"],"keywords":["Phospholipids","Virus–host Interactions","Viral Replication Complexes","Positive-strand Rna Viruses","Virus Control"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T11:07:07.021073Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}