{"doi":"10.1073/pnas.1432391100","title":"Functional reconstitution of COPI coat assembly and disassembly using chemically defined components","abstract":"<jats:p>\n            Coat protein I (COPI)-coated transport vesicles mediate protein and lipid\n transport in the early secretory pathway. The basic machinery required for the\n formation of these transport intermediates has been elucidated based on the\n reconstitution of COPI-coated vesicle formation from chemically defined\n liposomes. In this experimental system, the coat components coatomer and\n GTP-bound ADP-ribosylation factor (ARF), as well as p23 as a membrane-bound\n receptor for COPI coat proteins, were shown to be both necessary and\n sufficient to promote COPI-coated vesicle formation. Based on biochemical and\n ultrastructural analyses, we now demonstrate that the catalytic domain of\n ARF-GTPase-activating protein (GAP) alone is sufficient to initiate uncoating\n of liposome-derived COPI-coated vesicles. By contrast, ARF-GAP activity is not\n required for COPI coat assembly and, therefore, does not seem to represent an\n essential coat component of COPI vesicles as suggested recently [Yang, J. S.,\n Lee, S. Y., Gao, M., Bourgoin, S., Randazzo, P. A.,\n            <jats:italic>et al.</jats:italic>\n            (2002)\n            <jats:italic>J. Cell Biol.</jats:italic>\n            159, 69–78]. Thus, a complete round of COPI coat\n assembly and disassembly has been reconstituted with purified components\n defining the core machinery of COPI vesicle biogenesis.\n          </jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2003,"id":31164,"datarank":3.602718372110779,"base_score":4.406719247264253,"endowment":4.406719247264253,"self_citation_contribution":0.6610078870896381,"citation_network_contribution":2.9417104850211406,"self_endowment_contribution":0.6610078870896381,"citer_contribution":2.9417104850211406,"corpus_percentile":null,"corpus_rank":null,"citation_count":81,"citer_count":67,"citers_with_citation_signal":54,"citers_with_endowment":54,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":167636,"name":"Michael Schweikert","orcid":null,"position":1,"is_corresponding":false},{"id":120496,"name":"Felix T. Wieland","orcid":"0000-0002-3031-1833","position":2,"is_corresponding":false},{"id":122088,"name":"Walter Nickel","orcid":null,"position":3,"is_corresponding":false},{"id":167634,"name":"Constanze Reinhard","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.406719247264253,"endowment":4.406719247264253,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"12832619","pmcid":"PMC166215","openalex_id":"https://openalex.org/W2118515161","authors":[],"funders":[],"total_grants":0,"fwci":2.2627,"citation_percentile":0.87073482,"influential_citations":3,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":2},{"year":2014,"count":4},{"year":2015,"count":1},{"year":2017,"count":1},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2021,"count":5},{"year":2022,"count":4},{"year":2023,"count":3},{"year":2024,"count":1},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/166215","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc166215?pdf=render","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/166215","host_type":"repository"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.1432391100","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.1432391100","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/12832619","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC166215","host_type":"repository"}],"fields_of_study":["Cellular transport and secretion","Lipid Membrane Structure and Behavior","RNA Interference and Gene Delivery","Biology","Medicine","Chemistry"],"mesh_terms":["Animals","Cattle","Golgi Apparatus","Guanosine Triphosphate","Humans","Hydrolysis","Liposomes","Membrane Proteins","Rabbits","Recombinant Fusion Proteins","Protein Structure, Tertiary","Receptors, Cytoplasmic and Nuclear","Catalytic Domain","GTPase-Activating Proteins","ADP-Ribosylation Factors","Coat Protein Complex I","ADP-Ribosylation Factor 1","COP-Coated Vesicles","Macromolecular Substances"],"keywords":["COPI","COPII","Vesicle","Cell biology","Golgi apparatus","ADP ribosylation factor","Biogenesis","GTPase","Biology","Biophysics","Chemistry","Biochemistry","Secretory pathway","Membrane","Endoplasmic reticulum"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T06:38:18.421352Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}