{"doi":"10.1073/pnas.141222498","title":"Related homing endonucleases I-\n                    <i>Bmo</i>\n                    I and I-\n                    <i>Tev</i>\n                    I use different strategies to cleave homologous recognition sites","abstract":"<jats:p>\n                    A typical homing endonuclease initiates mobility of its group I intron by recognizing DNA both upstream and downstream of the intron insertion site of intronless alleles, preventing the endonuclease from binding and cleaving its own intron-containing allele. Here, we describe a GIY-YIG family homing endonuclease, I-\n                    <jats:italic>Bmo</jats:italic>\n                    I, that possesses an unusual recognition sequence, encompassing 1 base pair upstream but 38 base pairs downstream of the intron insertion site. I-\n                    <jats:italic>Bmo</jats:italic>\n                    I binds intron-containing and intronless substrates with equal affinity but can nevertheless discriminate between the two for cleavage. I-\n                    <jats:italic>Bmo</jats:italic>\n                    I is encoded by a group I intron that interrupts the thymidylate synthase (TS) gene (\n                    <jats:italic>thy</jats:italic>\n                    A) of\n                    <jats:italic>Bacillus mojavensis</jats:italic>\n                    s87-18. This intron resembles one inserted 21 nucleotides further downstream in a homologous TS gene (\n                    <jats:italic>td</jats:italic>\n                    ) of\n                    <jats:italic>Escherichia coli</jats:italic>\n                    phage T4. I-\n                    <jats:italic>Tev</jats:italic>\n                    I, the T4\n                    <jats:italic>td</jats:italic>\n                    intron-encoded GIY-YIG endonuclease, is very similar to I-\n                    <jats:italic>Bmo</jats:italic>\n                    I, but each endonuclease gene is inserted within a different position of its respective intron. Remarkably, I-\n                    <jats:italic>Tev</jats:italic>\n                    I and I-\n                    <jats:italic>Bmo</jats:italic>\n                    I bind a homologous stretch of TS-encoding DNA and cleave their intronless substrates in very similar positions. Our results suggest that each endonuclease has independently evolved the ability to distinguish intron-containing from intronless alleles while maintaining the same conserved recognition sequence centered on DNA-encoding active site residues of TS.\n                  </jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2001,"id":641581,"datarank":0.5955437870328184,"base_score":3.970291913552122,"endowment":3.970291913552122,"self_citation_contribution":0.5955437870328184,"citation_network_contribution":0.0,"self_endowment_contribution":0.5955437870328184,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":52,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1649793,"name":"David A. Shub","orcid":null,"position":1,"is_corresponding":false},{"id":996578,"name":"David R. Edgell","orcid":"0000-0003-3858-6150","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Related homing endonucleases I-\n                    <i>Bmo</i>\n                    I and I-\n                    <i>Tev</i>\n                    I use different strategies to cleave homologous recognition sites","abstract":"<jats:p>\n                    A typical homing endonuclease initiates mobility of its group I intron by recognizing DNA both upstream and downstream of the intron insertion site of intronless alleles, preventing the endonuclease from binding and cleaving its own intron-containing allele. Here, we describe a GIY-YIG family homing endonuclease, I-\n                    <jats:italic>Bmo</jats:italic>\n                    I, that possesses an unusual recognition sequence, encompassing 1 base pair upstream but 38 base pairs downstream of the intron insertion site. I-\n                    <jats:italic>Bmo</jats:italic>\n                    I binds intron-containing and intronless substrates with equal affinity but can nevertheless discriminate between the two for cleavage. I-\n                    <jats:italic>Bmo</jats:italic>\n                    I is encoded by a group I intron that interrupts the thymidylate synthase (TS) gene (\n                    <jats:italic>thy</jats:italic>\n                    A) of\n                    <jats:italic>Bacillus mojavensis</jats:italic>\n                    s87-18. This intron resembles one inserted 21 nucleotides further downstream in a homologous TS gene (\n                    <jats:italic>td</jats:italic>\n                    ) of\n                    <jats:italic>Escherichia coli</jats:italic>\n                    phage T4. I-\n                    <jats:italic>Tev</jats:italic>\n                    I, the T4\n                    <jats:italic>td</jats:italic>\n                    intron-encoded GIY-YIG endonuclease, is very similar to I-\n                    <jats:italic>Bmo</jats:italic>\n                    I, but each endonuclease gene is inserted within a different position of its respective intron. Remarkably, I-\n                    <jats:italic>Tev</jats:italic>\n                    I and I-\n                    <jats:italic>Bmo</jats:italic>\n                    I bind a homologous stretch of TS-encoding DNA and cleave their intronless substrates in very similar positions. Our results suggest that each endonuclease has independently evolved the ability to distinguish intron-containing from intronless alleles while maintaining the same conserved recognition sequence centered on DNA-encoding active site residues of TS.\n                  </jats:p>","is_dataset_classified":null,"base_score":3.970291913552122,"endowment":3.970291913552122,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11416170","pmcid":"PMC35440","openalex_id":"https://openalex.org/W2130064290","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM37746","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM44844","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM044844","title":null}],"total_grants":3,"fwci":1.6788,"citation_percentile":0.83834626,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2013,"count":1},{"year":2014,"count":2},{"year":2015,"count":1},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2024,"count":2},{"year":2025,"count":2}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://www.pnas.org/doi/pdf/10.1073/pnas.141222498","host_type":"journal"},{"url":"https://www.pnas.org/doi/pdf/10.1073/pnas.141222498","host_type":"publisher"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.141222498","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.141222498","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11416170","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/35440","host_type":"repository"}],"fields_of_study":["RNA and protein synthesis mechanisms","Bacteriophages and microbial interactions","Bacterial Genetics and Biotechnology","Amino Acid Sequence","Bacillus","Endodeoxyribonucleases","Escherichia coli","Evolution, Molecular","Introns","Molecular Sequence Data","Sequence Alignment","Substrate Specificity"],"mesh_terms":["Amino Acid Sequence","Bacillus","Endodeoxyribonucleases","Escherichia coli","Introns","Molecular Sequence Data","Substrate Specificity","Sequence Alignment","Evolution, Molecular"],"keywords":["Homing endonuclease","Intron","Endonuclease","Biology","Recognition sequence","Cleave","Group I catalytic intron","Gene","Genetics","DNA","Molecular biology","Restriction enzyme","RNA","RNA splicing"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Reduced inequalities"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-07T19:06:41.745038Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}