{"doi":"10.1073/pnas.1408677111","title":"Enhancement of protein expression by alphavirus replicons by designing self-replicating subgenomic RNAs","abstract":"<jats:title>Significance</jats:title>\n          <jats:p>One of the goals of modern molecular medicine is delivery and expression of heterologous genes in living organisms. RNA-based delivery vectors are a safer choice than DNA vectors, but they are prone to degradation and are highly dependent on efficient delivery methods. One of the ways to improve RNA vector performance is to increase the level of expression of the encoded proteins. We followed this approach and modified standard alphavirus replicon-based expression systems to make the transcribed subgenomic RNA additionally amplifiable by viral replication enzymes. Higher levels of subgenomic RNA synthesis increased the replicons’ expression efficiency at least 10-fold. Such replicons can be widely applied for development of efficient DNA and RNA vaccines and protein production in vitro.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2014,"id":31943,"datarank":2.2236703201975927,"base_score":4.02535169073515,"endowment":4.02535169073515,"self_citation_contribution":0.6038027536102726,"citation_network_contribution":1.6198675665873201,"self_endowment_contribution":0.6038027536102726,"citer_contribution":1.6198675665873201,"corpus_percentile":null,"corpus_rank":null,"citation_count":55,"citer_count":52,"citers_with_citation_signal":47,"citers_with_endowment":47,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":125572,"name":"Svetlana Atasheva","orcid":null,"position":1,"is_corresponding":false},{"id":170143,"name":"Alexander J. McAuley","orcid":null,"position":2,"is_corresponding":false},{"id":105300,"name":"Jessica A. Plante","orcid":"0000-0002-4768-7458","position":3,"is_corresponding":false},{"id":125411,"name":"Elena I. Frolova","orcid":"0000-0001-6523-9336","position":4,"is_corresponding":false},{"id":170145,"name":"David W. C. Beasley","orcid":null,"position":5,"is_corresponding":false},{"id":124134,"name":"Ilya Frolov","orcid":"0000-0002-8548-2517","position":6,"is_corresponding":false},{"id":125442,"name":"Dal Young Kim","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.02535169073515,"endowment":4.02535169073515,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25002490","pmcid":"PMC4115546","openalex_id":"https://openalex.org/W1987728748","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"R01AI070207","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01AI073301","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01AI095449","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"T32 AI060549","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"T32-AI060549","title":null}],"total_grants":5,"fwci":3.2802,"citation_percentile":0.91373883,"influential_citations":2,"citation_trend":[{"year":2015,"count":2},{"year":2016,"count":5},{"year":2017,"count":3},{"year":2018,"count":1},{"year":2019,"count":4},{"year":2020,"count":4},{"year":2021,"count":2},{"year":2022,"count":6},{"year":2023,"count":11},{"year":2024,"count":6},{"year":2025,"count":8},{"year":2026,"count":3}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://www.pnas.org/content/pnas/111/29/10708.full.pdf","host_type":"journal"},{"url":"https://www.pnas.org/content/pnas/111/29/10708.full.pdf","host_type":"BRONZE"},{"url":"https://www.pnas.org/content/pnas/111/29/10708.full.pdf","host_type":"publisher"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.1408677111","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.1408677111","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25002490","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4115546","host_type":"repository"}],"fields_of_study":["Mosquito-borne diseases and control","HIV Research and Treatment","Plant Virus Research Studies","Medicine","Biology","Alphavirus","Animals","Antibodies, Neutralizing","Encephalitis Virus, Venezuelan Equine","Gene Expression","Genetic Vectors","Genome, Viral","Green Fluorescent Proteins","Interferon-beta","Intracellular Space","Mice","Protein Biosynthesis","RNA Interference","RNA, Viral","Replicon","Viral Proteins","Virus Replication","West Nile virus"],"mesh_terms":["Alphavirus","Animals","Encephalitis Virus, Venezuelan Equine","Genetic Vectors","Replicon","RNA, Viral","Protein Biosynthesis","Viral Proteins","Virus Replication","West Nile virus","Gene Expression","Genome, Viral","Interferon-beta","RNA Interference","Intracellular Space","Green Fluorescent Proteins","Mice","Antibodies, Neutralizing"],"keywords":["Replicon","Subgenomic mRNA","Biology","Alphavirus","RNA","Venezuelan equine encephalitis virus","Heterologous","Virology","RNA-dependent RNA polymerase","Togaviridae","Viral replication","Internal ribosome entry site","Genetics","Molecular biology","Gene","Genome","Virus","Ribosome","Vaccines","Expression vectors"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T09:19:35.370003Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}