{"doi":"10.1073/pnas.1408301111","title":"Quantitative and stoichiometric analysis of the microRNA content of exosomes","abstract":"<jats:title>Significance</jats:title>\n                  <jats:p>Exosomes have been a subject of great interest in recent years, especially in the context of the microRNAs (miRNAs) that they contain. Exosome-mediated miRNA transfer between cells has been proposed to be a mechanism for intercellular signaling and exosome-associated miRNAs in biofluids have been suggested as potential minimally invasive biomarkers for multiple human disease states. Remarkably, we show here that most exosomes derived from standard preparations do not harbor many copies of miRNA molecules. These findings suggest a reevaluation of current models of the mechanism of exosome-mediated miRNA communication and indicate that stoichiometric analysis will be valuable for the study of other populations of extracellular vesicles and their associated RNAs as well.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2014,"id":33012,"datarank":13.440154513052704,"base_score":6.9957661563048505,"endowment":6.9957661563048505,"self_citation_contribution":1.0493649234457276,"citation_network_contribution":12.390789589606976,"self_endowment_contribution":1.0493649234457276,"citer_contribution":12.390789589606976,"corpus_percentile":null,"corpus_rank":null,"citation_count":1091,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":25,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":173599,"name":"Qing Kang","orcid":null,"position":1,"is_corresponding":false},{"id":137361,"name":"Ingrid K. Ruf","orcid":null,"position":2,"is_corresponding":false},{"id":173600,"name":"Hilary A. Briggs","orcid":null,"position":3,"is_corresponding":false},{"id":173601,"name":"Lucia N. Vojtech","orcid":null,"position":4,"is_corresponding":false},{"id":173602,"name":"Sean M. Hughes","orcid":null,"position":5,"is_corresponding":false},{"id":173603,"name":"Heather H. Cheng","orcid":null,"position":6,"is_corresponding":false},{"id":173604,"name":"Jason D. Arroyo","orcid":null,"position":7,"is_corresponding":false},{"id":173605,"name":"Emily K. Meredith","orcid":null,"position":8,"is_corresponding":false},{"id":173606,"name":"Emily N. Gallichotte","orcid":null,"position":9,"is_corresponding":false},{"id":173607,"name":"Era L. Pogosova-Agadjanyan","orcid":null,"position":10,"is_corresponding":false},{"id":173608,"name":"Colm Morrissey","orcid":null,"position":11,"is_corresponding":false},{"id":173609,"name":"Derek L. Stirewalt","orcid":null,"position":12,"is_corresponding":false},{"id":173610,"name":"Florian Hladik","orcid":null,"position":13,"is_corresponding":false},{"id":173611,"name":"Evan Y. Yu","orcid":null,"position":14,"is_corresponding":false},{"id":173612,"name":"Celestia S. Higano","orcid":null,"position":15,"is_corresponding":false},{"id":11667,"name":"Muneesh Tewari","orcid":null,"position":16,"is_corresponding":false},{"id":173598,"name":"John R. Chevillet","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":6.9957661563048505,"endowment":6.9957661563048505,"datacite_reuse_total":25,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25267620","pmcid":"PMC4205618","openalex_id":"https://openalex.org/W2073341747","authors":[],"funders":[{"funder_name":"NIDDK NIH HHS","grant_id":"R01 DK085714","title":null},{"funder_name":"NCI NIH HHS","grant_id":"P30 CA015704","title":null},{"funder_name":"NCI NIH HHS","grant_id":"P50 CA097186","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R21 AI095023","title":null},{"funder_name":"NCI NIH HHS","grant_id":"T32 CA009515","title":null},{"funder_name":"NCI NIH HHS","grant_id":"P50-CA-097186","title":null},{"funder_name":"NCI NIH HHS","grant_id":"CA-85859","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"DK-085714","title":null},{"funder_name":"NCI NIH 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vesicles in disease","MicroRNA in disease regulation","Circular RNAs in diseases","Biology","Medicine","Cell Line, Tumor","Exosomes","Gene Dosage","Humans","MicroRNAs","Models, Biological","Neoplasms"],"mesh_terms":["Humans","Models, Biological","Neoplasms","Gene Dosage","MicroRNAs","Cell Line, Tumor","Exosomes"],"keywords":["Microvesicles","Exosome","microRNA","Context (archaeology)","Biology","Extracellular vesicles","Computational biology","Cell biology","Genetics","Gene","Circulating","Microvesicle"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.15033070.v1","title":"Additional file 1 of Circulating extracellular vesicles from individuals at high-risk of lung cancer induce pro-tumorigenic conversion of stromal cells through transfer of miR-126 and miR-320","publisher":"figshare","resource_type":"Presentation"},{"doi":"10.6084/m9.figshare.15033070","title":"Additional file 1 of Circulating extracellular vesicles from individuals at high-risk of lung cancer induce 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