{"doi":"10.1073/pnas.1322254111","title":"Dynamic look at DNA unwinding by a replicative helicase","abstract":"<jats:title>Significance</jats:title>\n                  <jats:p>Precise replication of the genome is essential for maintaining the integrity of genetic information in all forms of life. A key step in this process is the unwinding of the DNA double helix, a subject of intensive research and debate. We took a multifaceted approach to study fundamental mechanisms of helicase function, using the E1 helicase from papillomavirus. Our findings reveal that E1 employs a strand exclusion mechanism to unwind DNA with the N-terminal side leading at the replication fork. Intriguingly, DNA unwinding by E1 is modulated by the origin-recognition domain, suggesting a previously unsuspected role for this domain in regulating helicase activity.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2014,"id":673250,"datarank":0.635115975689589,"base_score":4.23410650459726,"endowment":4.23410650459726,"self_citation_contribution":0.635115975689589,"citation_network_contribution":0.0,"self_endowment_contribution":0.635115975689589,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":68,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1759024,"name":"Salman Syed","orcid":null,"position":1,"is_corresponding":false},{"id":902139,"name":"Eric J. 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We took a multifaceted approach to study fundamental mechanisms of helicase function, using the E1 helicase from papillomavirus. Our findings reveal that E1 employs a strand exclusion mechanism to unwind DNA with the N-terminal side leading at the replication fork. Intriguingly, DNA unwinding by E1 is modulated by the origin-recognition domain, suggesting a previously unsuspected role for this domain in regulating helicase activity.</jats:p>","is_dataset_classified":null,"base_score":4.23410650459726,"endowment":4.23410650459726,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24550505","pmcid":"PMC3948270","openalex_id":"https://openalex.org/W2106741497","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI072345","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM065367","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R56 AI072345","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"AI072345","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM065367","title":null},{"funder_name":"Howard Hughes Medical Institute","grant_id":"","title":null},{"funder_name":"Howard Hughes Medical Institute","grant_id":"","title":null}],"total_grants":7,"fwci":3.7683,"citation_percentile":0.94340753,"influential_citations":0,"citation_trend":[{"year":2014,"count":6},{"year":2015,"count":4},{"year":2016,"count":9},{"year":2017,"count":10},{"year":2018,"count":5},{"year":2019,"count":10},{"year":2020,"count":3},{"year":2021,"count":11},{"year":2022,"count":4},{"year":2024,"count":2},{"year":2025,"count":4}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://www.pnas.org/content/pnas/111/9/E827.full.pdf","host_type":"journal"},{"url":"https://www.pnas.org/content/pnas/111/9/E827.full.pdf","host_type":"publisher"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.1322254111","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.1322254111","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24550505","host_type":"repository"},{"url":"https://repository.cshl.edu/id/eprint/29569/1/JoshuaTor%20and%20Stenlund%20PNAS%202014.pdf","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3948270","host_type":"repository"}],"fields_of_study":["DNA Repair Mechanisms","Epigenetics and DNA Methylation","Genomics and Chromatin Dynamics","Base Sequence","DNA","DNA Helicases","DNA-Binding Proteins","Fluorescence Resonance Energy Transfer","Models, Biological","Models, Molecular","Molecular Sequence Data","Mutagenesis, Site-Directed","Nucleic Acid Conformation","Spectrometry, Fluorescence","Viral Proteins"],"mesh_terms":["Base Sequence","DNA","DNA Helicases","DNA-Binding Proteins","Models, Biological","Models, Molecular","Molecular Sequence Data","Nucleic Acid Conformation","Spectrometry, Fluorescence","Viral Proteins","Mutagenesis, Site-Directed","Fluorescence Resonance Energy Transfer"],"keywords":["Helicase","Minichromosome maintenance","DNA replication","Control of chromosome duplication","Biology","RNA Helicase A","Replication protein A","DNA","Primase","Genetics","dnaB helicase","Cell biology","Replisome","Origin recognition complex","Computational biology","Eukaryotic DNA replication","DNA-binding protein","Gene","Transcription factor","RNA","ATPase","Molecular Motors"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T12:49:47.033184Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}