{"doi":"10.1073/pnas.1206839109","title":"ESCRT-III binding protein MITD1 is involved in cytokinesis and has an unanticipated PLD fold that binds membranes","abstract":"<jats:p>The endosomal sorting complexes required for transport (ESCRT) proteins have a critical function in abscission, the final separation of the daughter cells during cytokinesis. Here, we describe the structure and function of a previously uncharacterized ESCRT-III interacting protein, MIT-domain containing protein 1 (MITD1). Crystal structures of MITD1 reveal a dimer, with a microtubule-interacting and trafficking (MIT) domain at the N terminus and a unique, unanticipated phospholipase D-like (PLD) domain at the C terminus that binds membranes. We show that the MIT domain binds to a subset of ESCRT-III subunits and that this interaction mediates MITD1 recruitment to the midbody during cytokinesis. Depletion of MITD1 causes a distinct cytokinetic phenotype consistent with destabilization of the midbody and abscission failure. These results suggest a model whereby MITD1 coordinates the activity of ESCRT-III during abscission with earlier events in the final stages of cell division.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2012,"id":42253,"datarank":1.5186701886088996,"base_score":3.5553480614894135,"endowment":3.5553480614894135,"self_citation_contribution":0.5333022092234121,"citation_network_contribution":0.9853679793854875,"self_endowment_contribution":0.5333022092234121,"citer_contribution":0.9853679793854875,"corpus_percentile":null,"corpus_rank":null,"citation_count":34,"citer_count":31,"citers_with_citation_signal":28,"citers_with_endowment":28,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":42.5,"fair_percentile":21.459982409850483,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":201620,"name":"Monica Agromayor","orcid":"0000-0001-6477-1750","position":1,"is_corresponding":false},{"id":202493,"name":"Takayuki Obita","orcid":null,"position":2,"is_corresponding":false},{"id":202494,"name":"Olga Perisic","orcid":null,"position":3,"is_corresponding":false},{"id":202495,"name":"Anna Caballe","orcid":null,"position":4,"is_corresponding":false},{"id":202496,"name":"Magdalena Kloc","orcid":null,"position":5,"is_corresponding":false},{"id":202497,"name":"Meindert H. 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