{"doi":"10.1073/pnas.0914705107","title":"En masse in vitro functional profiling of the axonal mechanosensitivity of sensory neurons","abstract":"<jats:p>\n                    Perception of the environment relies on somatosensory neurons. Mechanosensory, proprioceptor and many nociceptor subtypes of these neurons have specific mechanosensitivity profiles to adequately differentiate stimulus patterns. Nevertheless, the cellular basis of differential mechanosensation remains largely elusive. Successful transduction of sensory information relies on the recruitment of sensory neurons and mechanosensation occurring at their peripheral axonal endings in vivo. Conspicuously, existing in vitro models aimed to decipher molecular mechanisms of mechanosensation test single sensory neuron somata at any one time. Here, we introduce a compartmental in vitro chamber design to deliver precisely controlled mechanical stimulation of sensory axons with synchronous real-time imaging of Ca\n                    <jats:sup>2+</jats:sup>\n                    transients in neuronal somata that reliably reflect action potential firing patterns. We report of three previously not characterized types of mechanosensitive neuron subpopulations with distinct intrinsic axonal properties tuned specifically to static indentation or vibration stimuli, showing that different classes of sensory neurons are tuned to specific types of mechanical stimuli. Primary receptor currents of vibration neurons display rapidly adapting conductance reliably detected for every single stimulus during vibration and are consistently converted into action potentials. This result allows for the characterization of two critical steps of mechanosensation in vivo: primary signal detection and signal conversion into specific action potential firing patterns in axons.\n                  </jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2010,"id":627972,"datarank":0.4335557636844247,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"self_citation_contribution":0.4335557636844247,"citation_network_contribution":0.0,"self_endowment_contribution":0.4335557636844247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":322367,"name":"Misha Zilberter","orcid":"0000-0001-7164-8312","position":1,"is_corresponding":false},{"id":38388,"name":"Sten Linnarsson","orcid":"0000-0002-3491-3444","position":2,"is_corresponding":false},{"id":1455144,"name":"Jens Hjerling-Leffler","orcid":null,"position":3,"is_corresponding":false},{"id":311467,"name":"Per Uhlén","orcid":"0000-0003-1446-1062","position":4,"is_corresponding":false},{"id":238262,"name":"Tibor Harkany","orcid":"0000-0002-6637-5900","position":5,"is_corresponding":false},{"id":1625664,"name":"Patrik Ernfors","orcid":null,"position":6,"is_corresponding":false},{"id":1625663,"name":"Dmitry Usoskin","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"En masse in vitro functional profiling of the axonal mechanosensitivity of sensory neurons","abstract":"<jats:p>\n                    Perception of the environment relies on somatosensory neurons. Mechanosensory, proprioceptor and many nociceptor subtypes of these neurons have specific mechanosensitivity profiles to adequately differentiate stimulus patterns. Nevertheless, the cellular basis of differential mechanosensation remains largely elusive. Successful transduction of sensory information relies on the recruitment of sensory neurons and mechanosensation occurring at their peripheral axonal endings in vivo. Conspicuously, existing in vitro models aimed to decipher molecular mechanisms of mechanosensation test single sensory neuron somata at any one time. Here, we introduce a compartmental in vitro chamber design to deliver precisely controlled mechanical stimulation of sensory axons with synchronous real-time imaging of Ca\n                    <jats:sup>2+</jats:sup>\n                    transients in neuronal somata that reliably reflect action potential firing patterns. We report of three previously not characterized types of mechanosensitive neuron subpopulations with distinct intrinsic axonal properties tuned specifically to static indentation or vibration stimuli, showing that different classes of sensory neurons are tuned to specific types of mechanical stimuli. Primary receptor currents of vibration neurons display rapidly adapting conductance reliably detected for every single stimulus during vibration and are consistently converted into action potentials. This result allows for the characterization of two critical steps of mechanosensation in vivo: primary signal detection and signal conversion into specific action potential firing patterns in axons.\n                  </jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20736349","pmcid":"PMC2941336","openalex_id":null,"authors":[],"funders":[{"funder_name":"European Research Council","grant_id":"232675","title":"Identification of a new mechanism of stem cell self-renewal; direct implications on self-repair and tumor initiating cells in the brain"},{"funder_name":"European Commission","grant_id":"223489","title":"Molecular mechanisms of neuronal restoration:  novel approaches for Parkinson's Disease"},{"funder_name":"European Commission","grant_id":"261063","title":"Charting the landscape of brain development by large-scale single-cell transcriptomics and phylogenetic lineage reconstruction"}],"total_grants":3,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2941336","host_type":"repository"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.0914705107","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.0914705107","host_type":""},{"url":"http://www.pnas.org/content/107/37/16336.full.pdf","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/20736349","host_type":""},{"url":"http://dx.doi.org/10.1073/pnas.0914705107","host_type":""},{"url":"https://dx.doi.org/10.1073/pnas.0914705107","host_type":""},{"url":"http://dx.doi.org/doi:10.1073/pnas.0914705107","host_type":""}],"fields_of_study":["0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Axons","Cells, Cultured","Animals","Mice","Calcium","Vibration","Sensory Receptor Cells"],"keywords":["Mice","Sensory Receptor Cells","Animals","Calcium","Vibration","Axons","Cells, Cultured"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T10:23:17.250997Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}