{"doi":"10.1073/pnas.0906390106","title":"Structural imperatives impose diverse evolutionary constraints on helical membrane proteins","abstract":"<jats:p>The amino acid sequences of transmembrane regions of helical membrane proteins are highly constrained, diverging at slower rates than their extramembrane regions and than water-soluble proteins. Moreover, helical membrane proteins seem to fall into fewer families than water-soluble proteins. The reason for the differential restrictions on sequence remains unexplained. Here, we show that the evolution of transmembrane regions is slowed by a previously unrecognized structural constraint: Transmembrane regions bury more residues than extramembrane regions and soluble proteins. This fundamental feature of membrane protein structure is an important contributor to the differences in evolutionary rate and to an increased susceptibility of the transmembrane regions to disease-causing single-nucleotide polymorphisms.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2009,"id":664455,"datarank":2.846951219437436,"base_score":4.31748811353631,"endowment":4.31748811353631,"self_citation_contribution":0.6476232170304466,"citation_network_contribution":2.1993280024069892,"self_endowment_contribution":0.6476232170304466,"citer_contribution":2.1993280024069892,"corpus_percentile":null,"corpus_rank":null,"citation_count":74,"citer_count":70,"citers_with_citation_signal":58,"citers_with_endowment":58,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1734964,"name":"Nathan H. 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Here, we show that the evolution of transmembrane regions is slowed by a previously unrecognized structural constraint: Transmembrane regions bury more residues than extramembrane regions and soluble proteins. This fundamental feature of membrane protein structure is an important contributor to the differences in evolutionary rate and to an increased susceptibility of the transmembrane regions to disease-causing single-nucleotide polymorphisms.</jats:p>","is_dataset_classified":null,"base_score":4.31748811353631,"endowment":4.31748811353631,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19815527","pmcid":"PMC2764890","openalex_id":"https://openalex.org/W2003037477","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM081783","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM063919","title":null}],"total_grants":2,"fwci":2.1427,"citation_percentile":0.86870485,"influential_citations":0,"citation_trend":[{"year":2012,"count":3},{"year":2013,"count":7},{"year":2014,"count":3},{"year":2015,"count":8},{"year":2016,"count":3},{"year":2017,"count":3},{"year":2018,"count":1},{"year":2019,"count":7},{"year":2020,"count":4},{"year":2021,"count":5},{"year":2022,"count":5},{"year":2023,"count":1},{"year":2024,"count":3},{"year":2025,"count":6},{"year":2026,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2764890","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2764890","host_type":"repository"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.0906390106","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.0906390106","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19815527","host_type":"repository"}],"fields_of_study":["Lipid Membrane Structure and Behavior","RNA and protein synthesis mechanisms","Protein Structure and Dynamics","Biophysical Phenomena","Databases, Protein","Evolution, Molecular","Humans","Hydrophobic and Hydrophilic Interactions","Membrane Proteins","Models, Molecular","Mutation","Polymorphism, Single Nucleotide","Protein Folding","Protein Structure, Secondary","Solubility"],"mesh_terms":["Humans","Membrane Proteins","Models, Molecular","Mutation","Solubility","Protein Structure, Secondary","Protein Folding","Evolution, Molecular","Polymorphism, Single Nucleotide","Databases, Protein","Biophysical Phenomena","Hydrophobic and Hydrophilic Interactions"],"keywords":["Transmembrane protein","Membrane protein","Transmembrane domain","Biology","Peptide sequence","Protein structure","Biochemistry","Amino acid","Biophysics","Computational biology","Membrane","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-13T03:00:11.108462Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}