{"doi":"10.1073/pnas.0906157106","title":"Single-molecule analysis reveals that the lagging strand increases replisome processivity but slows replication fork progression","abstract":"<jats:p>\n                    Single-molecule techniques are developed to examine mechanistic features of individual\n                    <jats:named-content content-type=\"genus-species\">E. coli</jats:named-content>\n                    replisomes during synthesis of long DNA molecules. We find that single replisomes exhibit constant rates of fork movement, but the rates of different replisomes vary over a surprisingly wide range. Interestingly, lagging strand synthesis decreases the rate of the leading strand, suggesting that lagging strand operations exert a drag on replication fork progression. The opposite is true for processivity. The lagging strand significantly increases the processivity of the replisome, possibly reflecting the increased grip to DNA provided by 2 DNA polymerases anchored to sliding clamps on both the leading and lagging strands.\n                  </jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2009,"id":603766,"datarank":0.7193685818395114,"base_score":4.795790545596741,"endowment":4.795790545596741,"self_citation_contribution":0.7193685818395114,"citation_network_contribution":0.0,"self_endowment_contribution":0.7193685818395114,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":120,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":293032,"name":"Roxana E. Georgescu","orcid":"0000-0002-1882-2358","position":1,"is_corresponding":false},{"id":1548865,"name":"Jeff Finkelstein","orcid":null,"position":2,"is_corresponding":false},{"id":1548866,"name":"Michael E. O'Donnell","orcid":null,"position":3,"is_corresponding":false},{"id":589648,"name":"Nina Y. Yao","orcid":"0000-0002-4728-6531","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Single-molecule analysis reveals that the lagging strand increases replisome processivity but slows replication fork progression","abstract":"<jats:p>\n                    Single-molecule techniques are developed to examine mechanistic features of individual\n                    <jats:named-content content-type=\"genus-species\">E. coli</jats:named-content>\n                    replisomes during synthesis of long DNA molecules. We find that single replisomes exhibit constant rates of fork movement, but the rates of different replisomes vary over a surprisingly wide range. Interestingly, lagging strand synthesis decreases the rate of the leading strand, suggesting that lagging strand operations exert a drag on replication fork progression. The opposite is true for processivity. The lagging strand significantly increases the processivity of the replisome, possibly reflecting the increased grip to DNA provided by 2 DNA polymerases anchored to sliding clamps on both the leading and lagging strands.\n                  </jats:p>","is_dataset_classified":null,"base_score":4.795790545596741,"endowment":4.795790545596741,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19666586","pmcid":"PMC2726342","openalex_id":"https://openalex.org/W1999350701","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM38839","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R37 GM038839","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM038839","title":null},{"funder_name":"Howard Hughes Medical Institute","grant_id":"","title":null},{"funder_name":"Howard Hughes Medical Institute","grant_id":"","title":null}],"total_grants":5,"fwci":2.8352,"citation_percentile":0.90644098,"influential_citations":0,"citation_trend":[{"year":2012,"count":8},{"year":2013,"count":11},{"year":2014,"count":8},{"year":2015,"count":8},{"year":2016,"count":9},{"year":2017,"count":12},{"year":2018,"count":8},{"year":2019,"count":9},{"year":2020,"count":5},{"year":2021,"count":9},{"year":2022,"count":10},{"year":2023,"count":3},{"year":2024,"count":1},{"year":2025,"count":3},{"year":2026,"count":3}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://www.pnas.org/content/pnas/106/32/13236.full.pdf","host_type":"journal"},{"url":"https://www.pnas.org/content/pnas/106/32/13236.full.pdf","host_type":"publisher"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.0906157106","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.0906157106","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19666586","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2726342","host_type":"repository"}],"fields_of_study":["DNA and Nucleic Acid Chemistry","DNA Repair Mechanisms","Advanced biosensing and bioanalysis techniques","DNA Polymerase III","DNA Replication","DNA, Circular","DNA-Directed DNA Polymerase","Diffusion","DnaB Helicases","Escherichia coli","Lipid Bilayers","Multienzyme Complexes","Replisomes"],"mesh_terms":["Replisomes","Diffusion","DNA Polymerase III","DNA-Directed DNA Polymerase","DNA Replication","DNA, Circular","Escherichia coli","Lipid Bilayers","Multienzyme Complexes","DnaB Helicases"],"keywords":["Replisome","Processivity","Biology","DNA replication","Lagging","DNA","Cell biology","Biophysics","Genetics","Eukaryotic DNA replication"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T22:40:18.450719Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}