{"doi":"10.1073/pnas.0902761106","title":"Hv1 proton channels are required for high-level NADPH oxidase-dependent superoxide production during the phagocyte respiratory burst","abstract":"<jats:p>\n                    Granulocytes generate a “respiratory burst” of NADPH oxidase-dependent superoxide anion (O\n                    <jats:sub arrange=\"stack\">2</jats:sub>\n                    <jats:sup arrange=\"stack\">−∙</jats:sup>\n                    ) production that is required for efficient clearance of bacterial pathogens. Hv1 mediates a voltage-gated H\n                    <jats:sup>+</jats:sup>\n                    channel activity that is proposed to serve a charge-balancing role in granulocytic phagocytes such as neutrophils and eosinophils. Using mice in which the gene encoding Hv1 is replaced by β-Geo reporter protein sequence, we show that Hv1 expression is required for measurable voltage-gated H\n                    <jats:sup>+</jats:sup>\n                    current in unstimulated phagocytes. O\n                    <jats:sub arrange=\"stack\">2</jats:sub>\n                    <jats:sup arrange=\"stack\">−∙</jats:sup>\n                    production is substantially reduced in the absence of Hv1, suggesting that Hv1 contributes a majority of the charge compensation required for optimal NADPH oxidase activity. Despite significant reduction in superoxide production,\n                    <jats:italic>Hv1</jats:italic>\n                    <jats:sup>−/−</jats:sup>\n                    mice are able to clear several types of bacterial infections.\n                  </jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2009,"id":596358,"datarank":0.8358231048266649,"base_score":5.572154032177765,"endowment":5.572154032177765,"self_citation_contribution":0.8358231048266649,"citation_network_contribution":0.0,"self_endowment_contribution":0.8358231048266649,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":262,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":4,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":656897,"name":"Evelyne Ruchti","orcid":"0000-0003-1211-2336","position":1,"is_corresponding":false},{"id":1527329,"name":"J. Stefan Kaczmarek","orcid":null,"position":2,"is_corresponding":false},{"id":150057,"name":"David E. Clapham","orcid":null,"position":3,"is_corresponding":false},{"id":436238,"name":"I. Scott Ramsey","orcid":"0000-0002-6432-4253","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Hv1 proton channels are required for high-level NADPH oxidase-dependent superoxide production during the phagocyte respiratory burst","abstract":"<jats:p>\n                    Granulocytes generate a “respiratory burst” of NADPH oxidase-dependent superoxide anion (O\n                    <jats:sub arrange=\"stack\">2</jats:sub>\n                    <jats:sup arrange=\"stack\">−∙</jats:sup>\n                    ) production that is required for efficient clearance of bacterial pathogens. Hv1 mediates a voltage-gated H\n                    <jats:sup>+</jats:sup>\n                    channel activity that is proposed to serve a charge-balancing role in granulocytic phagocytes such as neutrophils and eosinophils. Using mice in which the gene encoding Hv1 is replaced by β-Geo reporter protein sequence, we show that Hv1 expression is required for measurable voltage-gated H\n                    <jats:sup>+</jats:sup>\n                    current in unstimulated phagocytes. O\n                    <jats:sub arrange=\"stack\">2</jats:sub>\n                    <jats:sup arrange=\"stack\">−∙</jats:sup>\n                    production is substantially reduced in the absence of Hv1, suggesting that Hv1 contributes a majority of the charge compensation required for optimal NADPH oxidase activity. Despite significant reduction in superoxide production,\n                    <jats:italic>Hv1</jats:italic>\n                    <jats:sup>−/−</jats:sup>\n                    mice are able to clear several types of bacterial infections.\n                  </jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":4,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19372380","pmcid":null,"openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":19,"citation_trend":[],"oa_status":"green","license":null,"oa_locations":[{"url":"https://europepmc.org/articles/pmc2669790?pdf=render","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2669790","host_type":"repository"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.0902761106","host_type":"publisher"}],"fields_of_study":["Biology","Medicine"],"mesh_terms":[],"keywords":[],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.13120467.v1","title":"Additional file 2 of The voltage-gated proton channel Hv1 contributes to neuronal injury and motor deficits in a mouse model of spinal cord injury","publisher":"figshare","resource_type":"Image"},{"doi":"10.6084/m9.figshare.13120464","title":"Additional file 1 of The voltage-gated proton channel Hv1 contributes to neuronal injury and motor deficits in a mouse model of spinal cord injury","publisher":"figshare","resource_type":"Image"},{"doi":"10.6084/m9.figshare.13120467","title":"Additional file 2 of The voltage-gated proton channel Hv1 contributes to neuronal injury and motor deficits in a mouse model of spinal cord injury","publisher":"figshare","resource_type":"Image"},{"doi":"10.6084/m9.figshare.13120464.v1","title":"Additional file 1 of The voltage-gated proton channel Hv1 contributes to neuronal injury and motor deficits in a mouse model of spinal cord injury","publisher":"figshare","resource_type":"Image"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T10:00:42.836858Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}