{"doi":"10.1073/pnas.0802008105","title":"Differential requirements for Alix and ESCRT-III in cytokinesis and HIV-1 release","abstract":"<jats:p>The ESCRT machinery functions in topologically equivalent membrane fission events, namely multivesicular body formation, the terminal stages of cytokinesis and HIV-1 release. Here, we show that the ESCRT-III-binding protein Alix is recruited to the midbody of dividing cells through binding Cep55 via an evolutionarily conserved peptide. Disruption of Cep55/Alix/ESCRT-III interactions causes formation of aberrant midbodies and cytokinetic failure, demonstrating an essential role for these proteins in midbody morphology and cell division. We also show that the C terminus of Alix encodes a multimerization activity that is essential for its function in Alix-dependent HIV-1 release and for interaction with Tsg101. Last, we demonstrate that overexpression of Chmp4b and Chmp4c differentially inhibits HIV-1 release and cytokinesis, suggesting possible reasons for gene expansion within the mammalian Class E VPS pathway.</jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2008,"id":42228,"datarank":9.209147170556372,"base_score":5.424950017481403,"endowment":5.424950017481403,"self_citation_contribution":0.8137425026222106,"citation_network_contribution":8.395404667934162,"self_endowment_contribution":0.8137425026222106,"citer_contribution":8.395404667934162,"corpus_percentile":null,"corpus_rank":null,"citation_count":226,"citer_count":200,"citers_with_citation_signal":181,"citers_with_endowment":181,"datacite_reuse_total":12,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":201620,"name":"Monica Agromayor","orcid":"0000-0001-6477-1750","position":1,"is_corresponding":false},{"id":201621,"name":"Juan Martin-Serrano","orcid":"0000-0002-8183-3914","position":2,"is_corresponding":false},{"id":202415,"name":"Jez G. 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transport and secretion","HIV Research and Treatment","Phagocytosis and Immune Regulation","Biology","Medicine","Calcium-Binding Proteins","Cell Cycle Proteins","Cell Nucleus","Cytokinesis","DNA-Binding Proteins","Endosomal Sorting Complexes Required for Transport","Endosomes","Gene Expression Regulation","Gene Expression Regulation, Viral","HIV-1","HeLa Cells","Humans","Models, Biological","Nuclear Proteins","Protein Binding","Protein Structure, Tertiary","RNA, Small Interfering","Transcription Factors","Vesicular Transport Proteins","Tsg101 Protein"],"mesh_terms":["Tsg101 Protein","Calcium-Binding Proteins","Cell Nucleus","DNA-Binding Proteins","Gene Expression Regulation","HeLa Cells","Humans","Models, Biological","Nuclear Proteins","Protein Binding","Endosomes","Transcription Factors","HIV-1","Gene Expression Regulation, Viral","Protein Structure, Tertiary","Cell Cycle Proteins","Vesicular Transport Proteins","RNA, Small Interfering","Cytokinesis","Endosomal Sorting Complexes Required for Transport","Hela Cells"],"keywords":["ESCRT","Cytokinesis","Midbody","TSG101","Cell biology","Biology","Cell division","Chemistry","Cell","Endosome","Genetics","Gene","Intracellular","Microvesicles"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[{"doi":"10.6084/m9.figshare.14391198.v1","title":"Additional file 1 of The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.14391198","title":"Additional file 1 of The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.14391201.v1","title":"Additional file 2 of The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon 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