{"doi":"10.1073/pnas.0706974104","title":"Processing and function of CFTR-ΔF508 are species-dependent","abstract":"<jats:p>\n                    Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) cause cystic fibrosis. The most common mutation, a deletion of the phenylalanine at position 508 (ΔF508), disrupts processing of the protein. Nearly all human CFTR-ΔF508 is retained in the endoplasmic reticulum and degraded, preventing maturation to the plasma membrane. In addition, the F508 deletion reduces the activity of single CFTR channels. Human CFTR-ΔF508 has been extensively studied to better understand its defects. Here, we adopted a cross-species comparative approach, examining human, pig, and mouse CFTR-ΔF508. As with human CFTR-ΔF508, the ΔF508 mutation reduced the single-channel activity of the pig and mouse channels. However, the mutant pig and mouse proteins were at least partially processed like their wild-type counterparts. Moreover, pig and mouse CFTR-ΔF508 partially restored transepithelial Cl\n                    <jats:sup>−</jats:sup>\n                    transport to CF airway epithelia. Our data, combined with earlier work, suggest that there is a gradient in the severity of the CFTR-ΔF508 processing defect, with human more severe than pig or mouse. These findings may explain some previously puzzling observations in CF mice, they have important implications for evaluation of potential therapeutics, and they suggest new strategies for discovering the mechanisms that disrupt processing of human CFTR-ΔF508.\n                  </jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2007,"id":636621,"datarank":0.7130385286659547,"base_score":4.7535901911063645,"endowment":4.7535901911063645,"self_citation_contribution":0.7130385286659547,"citation_network_contribution":0.0,"self_endowment_contribution":0.7130385286659547,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":115,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":607014,"name":"Christopher S. Rogers","orcid":"0000-0002-5114-8801","position":1,"is_corresponding":false},{"id":134374,"name":"Qian Dong","orcid":"0000-0002-2766-2974","position":2,"is_corresponding":false},{"id":706230,"name":"Christoph O. Randak","orcid":"0000-0003-3264-4137","position":3,"is_corresponding":false},{"id":557350,"name":"Daniel W. Vermeer","orcid":"0000-0002-1501-0418","position":4,"is_corresponding":false},{"id":1652445,"name":"Tatiana Rokhlina","orcid":null,"position":5,"is_corresponding":false},{"id":248537,"name":"Philip H. Karp","orcid":null,"position":6,"is_corresponding":false},{"id":401889,"name":"Michael J. Welsh","orcid":"0000-0002-1646-6206","position":7,"is_corresponding":false},{"id":465063,"name":"Lynda S. Ostedgaard","orcid":"0000-0001-7717-3442","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Processing and function of CFTR-ΔF508 are species-dependent","abstract":"<jats:p>\n                    Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) cause cystic fibrosis. The most common mutation, a deletion of the phenylalanine at position 508 (ΔF508), disrupts processing of the protein. Nearly all human CFTR-ΔF508 is retained in the endoplasmic reticulum and degraded, preventing maturation to the plasma membrane. In addition, the F508 deletion reduces the activity of single CFTR channels. Human CFTR-ΔF508 has been extensively studied to better understand its defects. Here, we adopted a cross-species comparative approach, examining human, pig, and mouse CFTR-ΔF508. As with human CFTR-ΔF508, the ΔF508 mutation reduced the single-channel activity of the pig and mouse channels. However, the mutant pig and mouse proteins were at least partially processed like their wild-type counterparts. Moreover, pig and mouse CFTR-ΔF508 partially restored transepithelial Cl\n                    <jats:sup>−</jats:sup>\n                    transport to CF airway epithelia. Our data, combined with earlier work, suggest that there is a gradient in the severity of the CFTR-ΔF508 processing defect, with human more severe than pig or mouse. These findings may explain some previously puzzling observations in CF mice, they have important implications for evaluation of potential therapeutics, and they suggest new strategies for discovering the mechanisms that disrupt processing of human CFTR-ΔF508.\n                  </jats:p>","is_dataset_classified":null,"base_score":4.7535901911063645,"endowment":4.7535901911063645,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17873061","pmcid":"PMC1976592","openalex_id":"https://openalex.org/W2069109845","authors":[],"funders":[{"funder_name":"NHLBI NIH HHS","grant_id":"P01 HL051670","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"T32 HL007638","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"HL51670","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"HL61234","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"P30 DK054759","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"P50 HL061234","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"DK54759","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"HL07638","title":null}],"total_grants":8,"fwci":7.5623,"citation_percentile":0.97564947,"influential_citations":0,"citation_trend":[{"year":2012,"count":10},{"year":2013,"count":5},{"year":2014,"count":6},{"year":2015,"count":5},{"year":2016,"count":4},{"year":2017,"count":1},{"year":2018,"count":5},{"year":2019,"count":8},{"year":2020,"count":5},{"year":2021,"count":6},{"year":2022,"count":6},{"year":2024,"count":4},{"year":2025,"count":2},{"year":2026,"count":2}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1976592","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1976592","host_type":"repository"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.0706974104","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.0706974104","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17873061","host_type":"repository"}],"fields_of_study":["Cystic Fibrosis Research Advances","Neonatal Respiratory Health Research","Advanced biosensing and bioanalysis techniques"],"mesh_terms":["Animals","Biological Transport","Chlorocebus aethiops","Chlorine","Cystic Fibrosis","Electrophysiology","Endoplasmic Reticulum","Humans","Mutation","Phenylalanine","Species Specificity","Swine","Cystic Fibrosis Transmembrane Conductance Regulator","COS Cells","Mice"],"keywords":["ΔF508","Cystic fibrosis transmembrane conductance regulator","Cystic fibrosis","Chloride channel","Biology","Mutation","Endoplasmic reticulum","Mutant","Cell biology","Molecular biology","Genetics","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T17:24:16.833879Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}