{"doi":"10.1073/pnas.0600869103","title":"Acute suppression, but not chronic genetic deficiency, of c-\n                    <i>fos</i>\n                    gene expression impairs long-term memory in aversive taste learning","abstract":"<jats:p>\n                    Several lines of evidence have indicated that the establishment of long-term memory requires protein synthesis, including the synthesis of immediate-early gene products. Although the anatomical expression patterns of the c-\n                    <jats:italic>fos</jats:italic>\n                    gene, a transcription factor-encoding immediate-early gene, in conditioned taste aversion (CTA) are well documented, the functional roles of c-\n                    <jats:italic>fos</jats:italic>\n                    gene expression and Fos-mediated transcription remain to be clarified. Using the antisense oligodeoxynucleotide (AS-ODN) method in rats and gene-targeting knockout techniques in mice (c-\n                    <jats:italic>fos</jats:italic>\n                    <jats:sup>−/−</jats:sup>\n                    mice), we examined the roles of c-\n                    <jats:italic>fos</jats:italic>\n                    gene expression in the acquisition, retrieval, and retention of CTA. Preconditioning microinfusion of AS-ODN directed against c-\n                    <jats:italic>fos</jats:italic>\n                    mRNA (c-\n                    <jats:italic>fos</jats:italic>\n                    AS-ODN) into the parabrachial nucleus (PBN) impaired the acquisition, whereas infusion of control ODNs consisting of a randomized or inverted base order had no effect. Microinfusion of c-\n                    <jats:italic>fos</jats:italic>\n                    AS-ODN into either the amygdala or insular cortex did not impair the acquisition, whereas it attenuated the retention. Retrieval and subsequent retention of an acquired CTA were not disrupted by c-\n                    <jats:italic>fos</jats:italic>\n                    AS-ODN infusion into the PBN or amygdala. Microinfusion of another AS-ODN directed against\n                    <jats:italic>zif</jats:italic>\n                    268 (\n                    <jats:italic>egr</jats:italic>\n                    -1,\n                    <jats:italic>krox</jats:italic>\n                    -24,\n                    <jats:italic>NGFI</jats:italic>\n                    -A) mRNA into the PBN or amygdala did not affect the acquisition and retention. The genetic deficiency in c-\n                    <jats:italic>fos</jats:italic>\n                    <jats:sup>−/−</jats:sup>\n                    mice caused normal acquisition and retention. The present results suggest that the Fos-mediated gene transcription in the PBN, amygdala, or insular cortex plays critical roles in the acquisition and/or consolidation, but not the retrieval, of long-term taste memory; nevertheless, some other factors could compensate CTA mechanism when Fos-mediated transcription is not available.\n                  </jats:p>","journal":"Proceedings of the National Academy of Sciences","year":2006,"id":40781,"datarank":2.555512816932647,"base_score":4.330733340286331,"endowment":4.330733340286331,"self_citation_contribution":0.6496100010429497,"citation_network_contribution":1.9059028158896971,"self_endowment_contribution":0.6496100010429497,"citer_contribution":1.9059028158896971,"corpus_percentile":null,"corpus_rank":null,"citation_count":75,"citer_count":69,"citers_with_citation_signal":58,"citers_with_endowment":58,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":197535,"name":"Noritaka Sako","orcid":null,"position":1,"is_corresponding":false},{"id":175852,"name":"Emiko Senba","orcid":null,"position":2,"is_corresponding":false},{"id":92440,"name":"Takashi Yamamoto","orcid":"0000-0002-6516-2903","position":3,"is_corresponding":false},{"id":197534,"name":"Yasunobu Yasoshima","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.330733340286331,"endowment":4.330733340286331,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16636292","pmcid":"PMC1459025","openalex_id":"https://openalex.org/W2077904862","authors":[],"funders":[],"total_grants":0,"fwci":3.2713,"citation_percentile":0.92975856,"influential_citations":7,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":5},{"year":2014,"count":3},{"year":2015,"count":3},{"year":2016,"count":3},{"year":2017,"count":1},{"year":2018,"count":5},{"year":2019,"count":2},{"year":2020,"count":5},{"year":2021,"count":3},{"year":2022,"count":3},{"year":2023,"count":1},{"year":2026,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"http://doi.org/10.1073/pnas.0600869103","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc1459025?pdf=render","host_type":"GREEN"},{"url":"http://doi.org/10.1073/pnas.0600869103","host_type":"repository"},{"url":"https://pnas.org/doi/pdf/10.1073/pnas.0600869103","host_type":"publisher"},{"url":"https://doi.org/10.1073/pnas.0600869103","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16636292","host_type":"repository"},{"url":"http://europepmc.org/pmc/articles/PMC1459025","host_type":"repository"}],"fields_of_study":["Neuroscience and Neuropharmacology Research","Biochemical Analysis and Sensing Techniques","Memory and Neural Mechanisms","Biology","Medicine","Animals","Avoidance Learning","Behavior, Animal","Early Growth Response Protein 1","Gene Expression Regulation","Genes, fos","Male","Memory","Mice","Mice, Knockout","Neurons","Oligonucleotides, Antisense","Rats","Rats, Wistar","Taste","Transcription, Genetic"],"mesh_terms":["Animals","Avoidance Learning","Behavior, Animal","Gene Expression Regulation","Male","Memory","Neurons","Taste","Transcription, Genetic","Oligonucleotides, Antisense","Genes, fos","Rats, Wistar","Mice, Knockout","Mice","Rats","Early Growth Response Protein 1"],"keywords":["Immediate early gene","c-Fos","Amygdala","Taste aversion","Gene expression","Central nucleus of the amygdala","Neuroscience","Memory consolidation","Biology","Gene","Taste","Hippocampus","Genetics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-12T15:39:55.303385Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}