{"doi":"10.1063/5.0155207","title":"Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells","abstract":"During embryonic development, endothelial cells (ECs) undergo vasculogenesis to form a primitive plexus and assemble into networks comprised of mural cell-stabilized vessels with molecularly distinct artery and vein signatures. This organized vasculature is established prior to the initiation of blood flow and depends on a sequence of complex signaling events elucidated primarily in animal models, but less studied and understood in humans. Here, we have developed a simple vascular differentiation protocol for human pluripotent stem cells that generates ECs, pericytes, and smooth muscle cells simultaneously. When this protocol is applied in a 3D hydrogel, we demonstrate that it recapitulates the dynamic processes of early human vessel formation, including acquisition of distinct arterial and venous fates, resulting in a vasculogenesis angiogenesis model plexus (VAMP). The VAMP captures the major stages of vasculogenesis, angiogenesis, and vascular network formation and is a simple, rapid, scalable model system for studying early human vascular development in vitro.","journal":"APL Bioengineering","year":2023,"id":367280,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9433,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1124432,"name":"Shravani Kakarla","orcid":"0009-0005-7353-4518","position":1,"is_corresponding":false},{"id":904758,"name":"Max A. Winkelman","orcid":"0000-0003-2252-8212","position":2,"is_corresponding":false},{"id":627240,"name":"Keith Lane","orcid":null,"position":3,"is_corresponding":false},{"id":1124433,"name":"Katherine Stevens","orcid":"0009-0004-2388-0722","position":4,"is_corresponding":false},{"id":452094,"name":"Steven Lotz","orcid":null,"position":5,"is_corresponding":false},{"id":904757,"name":"Alexander Grath","orcid":"0000-0003-2812-1384","position":6,"is_corresponding":false},{"id":361884,"name":"Daylon James","orcid":"0000-0002-0975-2554","position":7,"is_corresponding":false},{"id":40564,"name":"Sally Temple","orcid":"0000-0001-7301-783X","position":8,"is_corresponding":false},{"id":287747,"name":"Guohao Dai","orcid":"0000-0001-7346-2685","position":9,"is_corresponding":false},{"id":40563,"name":"Taylor Bertucci","orcid":"0009-0008-1017-6807","position":0,"is_corresponding":true}],"reference_count":79,"raw_metadata":null,"created_at":"2026-07-19T01:15:12.215513Z","pmid":"37564277","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}