{"doi":"10.1055/a-2695-2258","title":"Effects of Osteochondral Allograft Reaming Protocols on Donor Chondrocyte Viability Prior to Transplantation","abstract":"Abstract This preclinical ex vivo study was conducted to evaluate the effects of submersion in saline or the Missouri Osteochondral Preservation System (MOPS®) solution during reaming on viable chondrocyte density (VCD) of osteochondral allografts (OCAs). Distal femoral OCAs preserved with MOPS were reamed to create cylindrical “plug” grafts using one of three techniques: Submersion in MOPS (SG–MOPS), submersion in saline (SG–Saline), or reamer saturated with MOPS without OCA submersion (SR–MOPS). All plug reaming was performed using standardized instrumentation and technique to harvest 18-mm-diameter plugs. Pre-reaming cartilage samples were collected to confirm baseline VCD. Post-reaming plugs were bisected and stained for live/dead analysis using fluorescent microscopy. VCD was quantified via image analysis, and %Day-0 VCD was calculated. Group comparisons were made using one-way analysis of variance (ANOVA; α = 0.05). A total of 21 plugs from 9 donors were analyzed: SG–MOPS (n = 8), SG–Saline (n = 6), SR–MOPS (n = 7). Mean %Day-0 VCD was highest in SG–MOPS (92.6 ± 7.8%), followed by SG–Saline (83.3 ± 10.2%), and SR–MOPS (80.2 ± 9.1%), though differences were not statistically significant (p = 0.68). A higher proportion of SG–MOPS plugs (7/8, 88%) exceeded the minimum essential VCD threshold (70%) compared with SG–Saline (4/6, 67%) and SR–MOPS (3/7, 43%). Submerging distal femur OCAs in MOPS during 18-mm-diameter femoral condyle plug reaming had clinically meaningful beneficial effects on viable donor chondrocyte density when compared with saline-submerged or non-submerged grafts. Based on the use of this submerged reaming technique that is standardized, repeatable, readily available, cost-effective, and safe, this methodology can be considered “best practice” for OCA plug reaming protocols, motivating implementation of this evidence-based shift in practice at our institution.","journal":"The Journal of Knee Surgery","year":2025,"id":574212,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9647,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":423954,"name":"Aaron M. Stoker","orcid":"0000-0001-6403-3969","position":1,"is_corresponding":false},{"id":423955,"name":"James P. Stannard","orcid":"0000-0001-8467-6494","position":2,"is_corresponding":false},{"id":1113361,"name":"Clayton W. Nuelle","orcid":"0000-0002-4859-224X","position":3,"is_corresponding":false},{"id":477880,"name":"Jacob S. Kramer","orcid":null,"position":4,"is_corresponding":false},{"id":1482003,"name":"Corder E. Lehenbauer","orcid":null,"position":5,"is_corresponding":false},{"id":325673,"name":"James L. Cook","orcid":"0000-0002-0862-995X","position":6,"is_corresponding":false},{"id":759764,"name":"Kylee Rucinski","orcid":"0000-0002-8627-1500","position":0,"is_corresponding":true}],"reference_count":20,"raw_metadata":null,"created_at":"2026-07-19T02:57:40.686992Z","pmid":"40907564","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}