{"doi":"10.1055/a-2318-0625","title":"Superoxide Anions Inhibit Intracellular Calcium Response in Porcine Airway Smooth Muscle Cells","abstract":"Abstract Background Superoxide anions (O2 −) have multiple effects on pulmonary parenchyma altering cell proliferation, cellular metabolism, and airway smooth muscle (ASM) contraction. Intracellular calcium ([Ca2+]i) concentration plays a significant role in the regulation of ASM contraction, relaxation, proliferation, and gene expression. Objective We investigated the effects of O2 − on agonist-stimulated changes in [Ca2+]i in ASM cells. Design/Methods Fura-2 AM-loaded, freshly isolated porcine ASM (PASM) cells were used to examine [Ca2+]i release in response to acetylcholine (ACh), histamine, endothelin, caffeine, and thapsigargin (TPG) in the presence or absence of extracellular Ca2+. Results Exposure of PASM cells to xanthine and xanthine oxidase (X + XO) resulted in a time-dependent generation of O2 −, inhibited by superoxide dismutase (SOD). Preincubating PASM cells with X + XO for 15- or 45-minute inhibited net [Ca2+]i responses to ACh, histamine, caffeine, and TPG compared with control cells. Pretreating PASM cells with SOD for 30 minutes mitigated the inhibitory effect of X + XO treatment on ACh-induced Ca2+ elevation suggesting role of O2 −. X + XO treatment also inhibited caffeine- and TPG-induced Ca2+ elevation suggesting effect of O2 − on [Ca2+]i release and reuptake mechanisms. Conclusion Superoxide attenuates [Ca2+]i release, reuptake, and may interfere with physiological functions of ASM cells.","journal":"研究報告集 II, 建築計画・都市計画・農村計画・建築経済・建築歴史・意匠","year":2024,"id":497306,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9588,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":402892,"name":"Mathur Kannan","orcid":"0000-0003-3624-4050","position":1,"is_corresponding":false},{"id":373525,"name":"Deepak A. Deshpande","orcid":"0000-0002-6187-5332","position":2,"is_corresponding":false},{"id":824832,"name":"Ramesh Krishnan","orcid":"0000-0003-1542-5127","position":0,"is_corresponding":true}],"reference_count":37,"raw_metadata":null,"created_at":"2026-07-19T02:09:30.779495Z","pmid":"38784940","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}