{"doi":"10.1046/j.1365-2958.2000.01791.x","title":"Non‐hydrolysable GTP‐γ‐S stabilizes the FtsZ polymer in a GDP‐bound state","abstract":"<jats:p>FtsZ, a tubulin homologue, forms a cytokinetic ring at the site of cell division in prokaryotes. The ring is thought to consist of polymers that assemble in a strictly GTP‐dependent way. GTP, but not guanosine‐5′‐<jats:italic>O</jats:italic>‐(3‐thiotriphosphate) (GTP‐γ‐S), has been shown to induce polymerization of FtsZ, whereas <jats:italic>in vitro</jats:italic> Ca<jats:sup>2+</jats:sup> is known to inhibit the GTP hydrolysis activity of FtsZ. We have studied FtsZ dynamics at limiting GTP concentrations in the presence of 10 mM Ca<jats:sup>2+</jats:sup>. GTP and its non‐hydrolysable analogue GTP‐γ‐S bind FtsZ with similar affinity, whereas the non‐hydrolysable analogue guanylyl‐imidodiphosphate (GMP‐PNP) is a poor substrate. Preformed FtsZ polymers can be stabilized by GTP‐γ‐S and are destabilized by GDP. As more than 95% of the nucleotide associated with the FtsZ polymer is in the GDP form, it is concluded that GTP hydrolysis by itself does not trigger FtsZ polymer disassembly. Strikingly, GTP‐γ‐S exchanges only a small portion of the FtsZ polymer‐bound GDP. These data suggest that FtsZ polymers are stabilized by a small fraction of GTP‐containing FtsZ subunits. These subunits may be located either throughout the polymer or at the polymer ends, forming a GTP cap similar to tubulin.</jats:p>","journal":"Molecular Microbiology","year":2000,"id":43009,"datarank":3.0587249761929423,"base_score":4.02535169073515,"endowment":4.02535169073515,"self_citation_contribution":0.6038027536102726,"citation_network_contribution":2.45492222258267,"self_endowment_contribution":0.6038027536102726,"citer_contribution":2.45492222258267,"corpus_percentile":null,"corpus_rank":null,"citation_count":55,"citer_count":46,"citers_with_citation_signal":41,"citers_with_endowment":41,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":205013,"name":"Tanneke Den Blaauwen","orcid":null,"position":1,"is_corresponding":false},{"id":186466,"name":"Arnold J. 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