{"doi":"10.1046/j.1365-2958.1997.4421810.x","title":"Requirements for ribosomal protein S1 for translation initiation of mRNAs with and without a 5′ leader sequence","abstract":"<jats:p>It has previously been proposed that <jats:italic>Escherichia coli</jats:italic> ribosomal protein S1 is required for the translation of highly structured mRNAs. In this study, we have examined the influence of structural features at or near the start codon of different mRNAs. The requirement for ribosomal protein S1 for translation initiation was determined when (i) the ribosome‐binding site (RBS) was either preceded by a 5′ non‐translated leader sequence; (ii) the RBS was located 5′ proximal to a mRNA start codon; and (iii) the start codon was the 5′ terminal codon as exemplified by leaderless mRNAs. <jats:italic>In vitro</jats:italic> translation studies revealed that the leaderless lambda <jats:italic>c</jats:italic>I mRNA is translated with <jats:italic>Bacillus stearothermophilus</jats:italic> ribosomes, naturally lacking a ribosomal protein S1 homologue, whereas <jats:italic>ompA</jats:italic> mRNA containing a 5′ leader is not. These studies have been verified by toeprinting with <jats:italic>E. coli</jats:italic> ribosomes depleted for S1. We have shown that S1 is required for ternary complex formation on <jats:italic>ompA</jats:italic> mRNA but not for leaderless mRNAs or for mRNAs in which the RBS is close to the 5′ end.</jats:p>","journal":"Molecular Microbiology","year":1997,"id":20913,"datarank":3.5371165916066394,"base_score":4.343805421853684,"endowment":4.343805421853684,"self_citation_contribution":0.6515708132780527,"citation_network_contribution":2.885545778328587,"self_endowment_contribution":0.6515708132780527,"citer_contribution":2.885545778328587,"corpus_percentile":null,"corpus_rank":null,"citation_count":76,"citer_count":63,"citers_with_citation_signal":55,"citers_with_endowment":55,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":136622,"name":"Armin Resch","orcid":null,"position":1,"is_corresponding":false},{"id":136623,"name":"Udo Bläsi","orcid":null,"position":2,"is_corresponding":false},{"id":136621,"name":"Karsten Tedin","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.343805421853684,"endowment":4.343805421853684,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11902720","pmcid":null,"openalex_id":"https://openalex.org/W2030979694","authors":[],"funders":[],"total_grants":0,"fwci":1.3497,"citation_percentile":0.80817209,"influential_citations":2,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":5},{"year":2014,"count":4},{"year":2015,"count":1},{"year":2016,"count":3},{"year":2017,"count":2},{"year":2018,"count":3},{"year":2019,"count":3},{"year":2020,"count":2},{"year":2021,"count":3},{"year":2022,"count":3},{"year":2023,"count":2},{"year":2024,"count":3},{"year":2025,"count":1}],"oa_status":"bronze","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1365-2958.1997.4421810.x","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1365-2958.1997.4421810.x","host_type":"BRONZE"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1365-2958.1997.4421810.x","host_type":"publisher"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2958.1997.4421810.x","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2958.1997.4421810.x","host_type":"publisher"},{"url":"https://onlinelibrary.wiley.com/doi/full-xml/10.1046/j.1365-2958.1997.4421810.x","host_type":"publisher"},{"url":"https://doi.org/10.1046/j.1365-2958.1997.4421810.x","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11902720","host_type":"repository"}],"fields_of_study":["RNA and protein synthesis mechanisms","RNA modifications and cancer","Viral Infections and Immunology Research","Biology","Medicine","5' Untranslated Regions","Bacterial Outer Membrane Proteins","Base Sequence","Escherichia coli","Geobacillus stearothermophilus","Molecular Sequence Data","Nucleic Acid Conformation","Protein Biosynthesis","RNA, Messenger","Ribosomal Proteins"],"mesh_terms":["Geobacillus stearothermophilus","Bacterial Outer Membrane Proteins","Base Sequence","Escherichia coli","Molecular Sequence Data","Nucleic Acid Conformation","Ribosomal Proteins","RNA, Messenger","Protein Biosynthesis","5' Untranslated Regions"],"keywords":["Biology","Ribosomal binding site","Start codon","Shine-Dalgarno sequence","Ribosome","Ribosomal protein","Messenger RNA","Ribosomal RNA","Translation (biology)","Molecular biology","30S","Eukaryotic translation","Protein biosynthesis","Eukaryotic Ribosome","Genetics","RNA","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-06T13:20:41.784303Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}