{"doi":"10.1046/j.1365-2583.1997.00151.x","title":"<b>Regulation of the ecdysone receptor, USP, E75 and MHR3 mRNAs by 20‐hydroxyecdysone in the GV1 cell line of the tobacco hornworm,</b>\n                    <b>\n                      <i>Manduca sexta</i>\n                    </b>","abstract":"<jats:p>\n                    <jats:bold>The responsiveness of several nuclear transcription factor genes to 20‐hydroxyecdysone (20E) was characterized in an embryonic cell line, GV1, from</jats:bold>\n                    <jats:bold>\n                      <jats:italic>Manduca sexta</jats:italic>\n                    </jats:bold>\n                    <jats:bold>. The mRNA for the</jats:bold>\n                    <jats:bold>\n                      <jats:italic>Manduca</jats:italic>\n                    </jats:bold>\n                    <jats:bold>ecdysone receptor (</jats:bold>\n                    <jats:bold>\n                      <jats:italic>Ms</jats:italic>\n                    </jats:bold>\n                    <jats:bold>\n                      EcR) was present in the GV1 cells and transiently increased 2.3‐fold by 5 h after the addition of 2 μg/ml (4 × 10\n                      <jats:sup>−6</jats:sup>\n                    </jats:bold>\n                    <jats:sc>M</jats:sc>\n                    <jats:bold>) 20‐hydroxyecdysone (20E). In contrast,</jats:bold>\n                    <jats:bold>\n                      <jats:italic>Manduca</jats:italic>\n                    </jats:bold>\n                    <jats:bold>ultraspiracle (</jats:bold>\n                    <jats:bold>\n                      <jats:italic>Ms</jats:italic>\n                    </jats:bold>\n                    <jats:bold>USP) mRNA level in the GV1 cells decreased slowly to half of its initial level by 12 h when exposed to the same concentration of 20E. The mRNAs for two putative transcription factors,</jats:bold>\n                    <jats:bold>\n                      <jats:italic>Ms</jats:italic>\n                    </jats:bold>\n                    <jats:bold>\n                      E75 and MHR3, were induced in the GV1 cells by 20E, with that for E75 appearing within 1 h whereas that for MHR3 within 2 h. The ED\n                      <jats:sub>50</jats:sub>\n                      s for induction of\n                    </jats:bold>\n                    <jats:bold>\n                      <jats:italic>MsE75</jats:italic>\n                    </jats:bold>\n                    <jats:bold>and</jats:bold>\n                    <jats:bold>\n                      <jats:italic>MHR3</jats:italic>\n                    </jats:bold>\n                    <jats:bold>\n                      gene expression in GV1 cells were 1.2 × 10\n                      <jats:sup>−6</jats:sup>\n                      and 2.5 × 10\n                      <jats:sup>−6</jats:sup>\n                    </jats:bold>\n                    <jats:sc>M</jats:sc>\n                    <jats:bold>20E, respectively.</jats:bold>\n                  </jats:p>","journal":"Insect Molecular Biology","year":1997,"id":32690,"datarank":1.9249309458875785,"base_score":3.6635616461296463,"endowment":3.6635616461296463,"self_citation_contribution":0.5495342469194471,"citation_network_contribution":1.3753966989681314,"self_endowment_contribution":0.5495342469194471,"citer_contribution":1.3753966989681314,"corpus_percentile":null,"corpus_rank":null,"citation_count":38,"citer_count":29,"citers_with_citation_signal":28,"citers_with_endowment":28,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":27125,"name":"Z. 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