{"doi":"10.1042/bst0370450","title":"Genomic DNA amplification by the multiple displacement amplification (MDA) method","abstract":"<jats:p>Large amounts of DNA are frequently required for use in detection assays and genomic analysis. The limited availability of DNA can be a critical obstacle to meeting research and clinical needs. DNA amplification methods are often required to generate sufficient material from small specimens or environmental samples with low DNA content. The MDA (multiple displacement amplification) reaction is increasingly the method of choice for many applications because of its extensive coverage of the genome, the generation of extremely long DNA products compared with older whole genome amplification methods and the high DNA yields, even from exceedingly low amounts of starting material. Remarkably, MDA enables genomic sequencing even from single microbial cells. Some of the uses of MDA and its strengths and limitations will be discussed.</jats:p>","journal":"Biochemical Society Transactions","year":2009,"id":612450,"datarank":0.7535820781269416,"base_score":5.0238805208462765,"endowment":5.0238805208462765,"self_citation_contribution":0.7535820781269416,"citation_network_contribution":0.0,"self_endowment_contribution":0.7535820781269416,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":151,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":2,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1576915,"name":"Roger S. Lasken","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Genomic DNA amplification by the multiple displacement amplification (MDA) method","abstract":"<jats:p>Large amounts of DNA are frequently required for use in detection assays and genomic analysis. The limited availability of DNA can be a critical obstacle to meeting research and clinical needs. DNA amplification methods are often required to generate sufficient material from small specimens or environmental samples with low DNA content. The MDA (multiple displacement amplification) reaction is increasingly the method of choice for many applications because of its extensive coverage of the genome, the generation of extremely long DNA products compared with older whole genome amplification methods and the high DNA yields, even from exceedingly low amounts of starting material. Remarkably, MDA enables genomic sequencing even from single microbial cells. Some of the uses of MDA and its strengths and limitations will be discussed.</jats:p>","is_dataset_classified":null,"base_score":5.0238805208462765,"endowment":5.0238805208462765,"datacite_reuse_total":2,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19290880","pmcid":null,"openalex_id":"https://openalex.org/W2091245185","authors":[],"funders":[],"total_grants":0,"fwci":10.9026,"citation_percentile":0.98793328,"influential_citations":7,"citation_trend":[{"year":2012,"count":13},{"year":2013,"count":6},{"year":2014,"count":12},{"year":2015,"count":12},{"year":2016,"count":6},{"year":2017,"count":8},{"year":2018,"count":12},{"year":2019,"count":6},{"year":2020,"count":7},{"year":2021,"count":1},{"year":2022,"count":14},{"year":2023,"count":11},{"year":2024,"count":9},{"year":2025,"count":6},{"year":2026,"count":4}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://portlandpress.com/biochemsoctrans/article-pdf/37/2/450/543770/bst0370450.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1042/bst0370450","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19290880","host_type":"repository"}],"fields_of_study":["Bacteriophages and microbial interactions","Genomics and Phylogenetic Studies","Chromosomal and Genetic Variations","Biology","Medicine","Chemistry"],"mesh_terms":["DNA","Genotype","Humans","Genome, Human","Genome, Bacterial","Nucleic Acid Amplification Techniques"],"keywords":["Multiple displacement amplification","genomic DNA","DNA","Genome","Computational biology","Biology","DNA sequencing","Genomics","Genetics","DNA extraction","Polymerase chain reaction","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[{"doi":"10.6084/m9.figshare.19211803.v1","title":"Additional file 1 of Multidisciplinary management for Peutz–Jeghers syndrome and prevention of vertical transmission to offspring using preimplantation genetic testing","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.19211803","title":"Additional file 1 of Multidisciplinary management for Peutz–Jeghers syndrome and prevention of vertical transmission to offspring using preimplantation genetic testing","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T03:29:47.471475Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}