{"doi":"10.1042/bsr20211693","title":"Mitochondrial DNA maintenance in <i>Drosophila melanogaster</i>","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>All 37 mitochondrial DNA (mtDNA)-encoded genes involved with oxidative phosphorylation and intramitochondrial protein synthesis, and several nuclear-encoded genes involved with mtDNA replication, transcription, repair and recombination are conserved between the fruit fly Drosophila melanogaster and mammals. This, in addition to its easy genetic tractability, has made Drosophila a useful model for our understanding of animal mtDNA maintenance and human mtDNA diseases. However, there are key differences between the Drosophila and mammalian systems that feature the diversity of mtDNA maintenance processes inside animal cells. Here, we review what is known about mtDNA maintenance in Drosophila, highlighting areas for which more research is warranted and providing a perspective preliminary in silico and in vivo analyses of the tissue specificity of mtDNA maintenance processes in this model organism. Our results suggest new roles (or the lack thereof) for well-known maintenance proteins, such as the helicase Twinkle and the accessory subunit of DNA polymerase γ, and for other Drosophila gene products that may even aid in shedding light on mtDNA maintenance in other animals. We hope to provide the reader some interesting paths that can be taken to help our community show how Drosophila may impact future mtDNA maintenance research.</jats:p>","journal":"Bioscience Reports","year":2022,"id":627672,"datarank":0.3453877639491069,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.0,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1624614,"name":"Audrey C. Novaes","orcid":null,"position":1,"is_corresponding":false},{"id":599645,"name":"Grzegorz L. Ciesielski","orcid":"0000-0001-6528-6781","position":2,"is_corresponding":false},{"id":778214,"name":"Marcos T. Oliveira","orcid":"0000-0003-4779-5755","position":3,"is_corresponding":false},{"id":1624613,"name":"Ana P.C. Rodrigues","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Mitochondrial DNA maintenance in <i>Drosophila melanogaster</i>","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>All 37 mitochondrial DNA (mtDNA)-encoded genes involved with oxidative phosphorylation and intramitochondrial protein synthesis, and several nuclear-encoded genes involved with mtDNA replication, transcription, repair and recombination are conserved between the fruit fly Drosophila melanogaster and mammals. This, in addition to its easy genetic tractability, has made Drosophila a useful model for our understanding of animal mtDNA maintenance and human mtDNA diseases. However, there are key differences between the Drosophila and mammalian systems that feature the diversity of mtDNA maintenance processes inside animal cells. Here, we review what is known about mtDNA maintenance in Drosophila, highlighting areas for which more research is warranted and providing a perspective preliminary in silico and in vivo analyses of the tissue specificity of mtDNA maintenance processes in this model organism. Our results suggest new roles (or the lack thereof) for well-known maintenance proteins, such as the helicase Twinkle and the accessory subunit of DNA polymerase γ, and for other Drosophila gene products that may even aid in shedding light on mtDNA maintenance in other animals. We hope to provide the reader some interesting paths that can be taken to help our community show how Drosophila may impact future mtDNA maintenance research.</jats:p>","is_dataset_classified":null,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36254835","pmcid":"PMC9653094","openalex_id":"https://openalex.org/W4306694283","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R15 GM139104","title":null},{"funder_name":"National Institutes of Health","grant_id":"3R15GM139104-01A1S1","title":"The Mechanism of Elimination of the Mitochondrial DNA Replisome"}],"total_grants":2,"fwci":0.549,"citation_percentile":0.61100916,"influential_citations":0,"citation_trend":[{"year":2022,"count":1},{"year":2024,"count":6},{"year":2026,"count":1}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://portlandpress.com/bioscirep/article-pdf/42/11/BSR20211693/939312/bsr-2021-1693c.pdf","host_type":"journal"},{"url":"https://portlandpress.com/bioscirep/article-pdf/42/11/BSR20211693/939312/bsr-2021-1693c.pdf","host_type":"publisher"},{"url":"https://portlandpress.com/bioscirep/article-pdf/doi/10.1042/BSR20211693/939312/bsr-2021-1693c.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1042/bsr20211693","host_type":"journal"},{"url":"https://dialnet.unirioja.es/servlet/articulo?codigo=1041638","host_type":"repository"},{"url":"https://pubmed.ncbi.nlm.nih.gov/36254835","host_type":"repository"},{"url":"https://digitalcommons.unf.edu/unf_faculty_publications/3276","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9653094","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC9653094","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC9653094?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1042/BSR20211693","host_type":""},{"url":"https://doi.org/https://doi.org/10.1042/BSR20211693","host_type":""}],"fields_of_study":["Mitochondrial Function and Pathology","ATP Synthase and ATPases Research","Photosynthetic Processes and Mechanisms","0301 basic medicine","03 medical and health sciences","0303 health sciences"],"mesh_terms":["DNA Polymerase gamma","Animals","DNA Replication","DNA, Mitochondrial","Drosophila melanogaster","Humans","Mammals","Mitochondria","Mitochondrial Proteins","Drosophila Proteins"],"keywords":["Drosophila melanogaster","Mitochondrial DNA","Biology","Genetics","Model organism","Gene","Drosophila (subgenus)","Genome","Cell biology","Computational biology","Mitochondria","Nucleic acids","Dna Synthesis And Repair","DNA Replication","DNA Polymerase gamma (genetics","DNA Replication (genetics)","Drosophila Proteins (metabolism)","Mammals (metabolism)","DNA, Mitochondrial","Mitochondrial Proteins","Mitochondria (genetics","Animals","Humans","Drosophila Proteins","Mitochondrial Proteins (genetics)","DNA, Chromosomes & Chromosomal Structure","Mammals","metabolism)","DNA","Drosophila melanogaster (genetics","DNA Polymerase gamma","Mitochondrial (genetics)"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T18:26:00.497431Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}