{"doi":"10.1042/bj3110251","title":"Identification of a functional promoter element in the 5′-flanking region of the rat cMG1/TIS11b gene","abstract":"<jats:p>The cMG1 gene was originally identified as a mitogen-stimulated primary response gene. However, in contrast to genes such as c-fos and TIS11, cMG1 is also expressed at significant levels before and after the transient elevation induced by agonists. We have sequenced a 1.3 kb rat genomic cMG1 clone, which includes 931 bp upstream of the transcription start site identified by primer-extension analysis. A 1033 bp fragment, including this 5′-flanking sequence, directed the expression of the reporter gene chloramphenicol acetyltransferase (CAT) in transfected NIH-3T3 cells. Progressive 5‘-to-3’ deletion indicated that an element located between -138 and -114 was responsible for most of this basal CAT expression. DNA mobility-shift assays showed that the sequence between -143 and -105 contained binding sites for cellular proteins, the principal complexes involving nucleotides between -119 and -105. We conclude that these complexes may represent the transcription factor-DNA element interactions that determine basal cMG1 expression.</jats:p>","journal":"Biochemical Journal","year":1995,"id":20794,"datarank":0.39240986852710846,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.1509941816619934,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.1509941816619934,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":3,"citers_with_citation_signal":3,"citers_with_endowment":3,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":136269,"name":"J C Pascall","orcid":null,"position":1,"is_corresponding":false},{"id":136270,"name":"K M Hadfield","orcid":null,"position":2,"is_corresponding":false},{"id":136271,"name":"K D Brown","orcid":null,"position":3,"is_corresponding":false},{"id":136268,"name":"A N Corps","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7575462","pmcid":"PMC1136146","openalex_id":"https://openalex.org/W2401038290","authors":[],"funders":[],"total_grants":0,"fwci":0.1355,"citation_percentile":0.47674214,"influential_citations":0,"citation_trend":[{"year":2016,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://portlandpress.com/biochemj/article-pdf/311/1/251/618112/bj3110251.pdf","host_type":"journal"},{"url":"https://europepmc.org/articles/pmc1136146?pdf=render","host_type":"GREEN"},{"url":"https://portlandpress.com/biochemj/article-pdf/311/1/251/618112/bj3110251.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1042/bj3110251","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7575462","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1136146","host_type":"repository"}],"fields_of_study":["Cancer-related gene regulation","Estrogen and related hormone effects","RNA Research and Splicing","Biology","Medicine","3T3 Cells","Animals","Base Sequence","Butyrate Response Factor 1","Chloramphenicol O-Acetyltransferase","DNA, Complementary","DNA-Binding Proteins","Gene Deletion","Gene Expression","Genes, Reporter","Immediate-Early Proteins","Mice","Molecular Sequence Data","PC12 Cells","Promoter Regions, Genetic","Proteins","RNA, Messenger","Rats","Sequence Analysis, DNA","Transfection","Tristetraprolin"],"mesh_terms":["Animals","Base Sequence","DNA-Binding Proteins","Molecular Sequence Data","Promoter Regions, Genetic","Proteins","RNA, Messenger","Transfection","Chloramphenicol O-Acetyltransferase","Gene Expression","3T3 Cells","PC12 Cells","Gene Deletion","Sequence Analysis, DNA","Immediate-Early Proteins","Genes, Reporter","DNA, Complementary","Mice","Rats","Tristetraprolin","Butyrate Response Factor 1"],"keywords":["Chloramphenicol acetyltransferase","5' flanking region","Molecular biology","Biology","Gene","Reporter gene","Response element","Regulatory sequence","Gene expression","Transcription (linguistics)","Primer extension","Transfection","Transcription factor","genomic DNA","Promoter","DNA","Regulation of gene expression","Genetics","Messenger RNA"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-06T11:27:26.855286Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}