{"doi":"10.1042/bj3090385","title":"Evaluation of the use of the luciferase-reporter-gene system for gene-regulation studies involving cyclic AMP-elevating agents","abstract":"<jats:p>The effects of cyclic AMP (cAMP)-elevating agents on the activity of cis-acting gene promoter sequences are frequently studied using the luciferase-reporter-gene system. The aim of the present study was to assess whether cAMP-elevating agents induce any changes in the level of luciferase activity independently of a transcriptional activation of promoter elements. Chloramphenicol acteyltransferase (CAT) and luciferase reporter genes under the control of the same promoter elements were transiently expressed in primary cultures of human vascular smooth-muscle cells. Transfected cells were treated with a cell-permeable and non-hydrolysable cAMP analogue, 2′-O-monobutyryl-8-bromo cAMP, or with the cAMP-elevating agents forskolin and prostaglandin E1 (PGE1). Forskolin and PGE1 induced a significant increase in the level of luciferase activity, but had no effect on CAT activity. Conclusions based solely on the use of the luciferase-reporter-gene system in studies involving promoter activation by cAMP-elevating agents could therefore be misleading.</jats:p>","journal":"Biochemical Journal","year":1995,"id":21292,"datarank":1.6829123145988247,"base_score":2.70805020110221,"endowment":2.70805020110221,"self_citation_contribution":0.40620753016533157,"citation_network_contribution":1.2767047844334931,"self_endowment_contribution":0.40620753016533157,"citer_contribution":1.2767047844334931,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":12,"citers_with_citation_signal":11,"citers_with_endowment":11,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":137647,"name":"C Kanthou","orcid":null,"position":1,"is_corresponding":false},{"id":137648,"name":"U Dennehy","orcid":null,"position":2,"is_corresponding":false},{"id":137649,"name":"A al Haq","orcid":null,"position":3,"is_corresponding":false},{"id":137650,"name":"L P Berg","orcid":null,"position":4,"is_corresponding":false},{"id":137651,"name":"V V Kakkar","orcid":null,"position":5,"is_corresponding":false},{"id":137652,"name":"D N Cooper","orcid":null,"position":6,"is_corresponding":false},{"id":137646,"name":"O Benzakour","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.70805020110221,"endowment":2.70805020110221,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7626000","pmcid":"PMC1135743","openalex_id":"https://openalex.org/W1692571807","authors":[],"funders":[],"total_grants":0,"fwci":0.271,"citation_percentile":0.49912503,"influential_citations":0,"citation_trend":[],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://portlandpress.com/biochemj/article-pdf/309/2/385/617222/bj3090385.pdf","host_type":"journal"},{"url":"https://europepmc.org/articles/pmc1135743?pdf=render","host_type":"GREEN"},{"url":"https://portlandpress.com/biochemj/article-pdf/309/2/385/617222/bj3090385.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1042/bj3090385","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7626000","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1135743","host_type":"repository"},{"url":"http://www.biochemj.org/bj/309/0385/3090385.pdf","host_type":"repository"}],"fields_of_study":["bioluminescence and chemiluminescence research","Viral Infectious Diseases and Gene Expression in Insects","Neurobiology and Insect Physiology Research","Biology","Medicine","Chemistry","8-Bromo Cyclic Adenosine Monophosphate","Adolescent","Adult","Alprostadil","Cells, Cultured","Child","Chloramphenicol O-Acetyltransferase","Colforsin","Cyclic AMP","Gene Expression Regulation, Enzymologic","Genes, Reporter","Humans","Luciferases","Middle Aged","Muscle, Smooth, Vascular"],"mesh_terms":["Cyclic AMP","Adolescent","Adult","Alprostadil","Cells, Cultured","Child","Colforsin","Humans","Luciferases","Middle Aged","Muscle, Smooth, Vascular","8-Bromo Cyclic Adenosine Monophosphate","Chloramphenicol O-Acetyltransferase","Gene Expression Regulation, Enzymologic","Genes, Reporter"],"keywords":["Luciferase","Reporter gene","Forskolin","Transfection","Gene","Promoter","Regulation of gene expression","Gene expression","Biology","Molecular biology","Chemistry","Cell biology","Cell culture","Biochemistry","Genetics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-06T15:20:32.071330Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}