{"doi":"10.1042/bj3020759","title":"Organization of the 5′ region of the rat ATP citrate lyase gene","abstract":"<jats:p>A genomic clone, encompassing the 5′ flanking region and the first seven exons of rat ATP citrate lyase gene, was isolated from a rat genomic library and sequenced. Primer-extension analysis showed that mRNA is transcribed at 4407 nucleotides upstream from the translation start site. Primer-extension analysis and sequencing of ATP citrate lyase cDNA amplified by PCR showed that the promoter used for transcription is identical in mammary gland, lung, liver, brain and kidney. Southern-blot analysis showed that the ATP citrate lyase gene exists as a single copy. The 5′ flanking region contains several consensus sequences defined as promoter elements. These include a CAAT box and Sp1-binding sites. However, a TATA box lacks this promoter. The expression of the chloramphenicol acetyltransferase gene was induced by the 5‘ flanking region (-2370 to -1) in the CHO cell line. The 5′ flanking region also contains several sequence elements that may be involved in the transcriptional regulation of the gene.</jats:p>","journal":"Biochemical Journal","year":1994,"id":20202,"datarank":0.957263736158569,"base_score":2.639057329615259,"endowment":2.639057329615259,"self_citation_contribution":0.3958585994422889,"citation_network_contribution":0.56140513671628,"self_endowment_contribution":0.3958585994422889,"citer_contribution":0.56140513671628,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":8,"citers_with_citation_signal":7,"citers_with_endowment":7,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":134353,"name":"S W Park","orcid":null,"position":1,"is_corresponding":false},{"id":134354,"name":"Y A Moon","orcid":null,"position":2,"is_corresponding":false},{"id":134355,"name":"Y S Kim","orcid":null,"position":3,"is_corresponding":false},{"id":134351,"name":"K S Kim","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.639057329615259,"endowment":2.639057329615259,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7945200","pmcid":"PMC1137296","openalex_id":"https://openalex.org/W2379468174","authors":[],"funders":[],"total_grants":0,"fwci":0.4438,"citation_percentile":0.65531136,"influential_citations":1,"citation_trend":[{"year":2014,"count":1}],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://ir.ymlib.yonsei.ac.kr/bitstream/22282913/195226/1/T199401692.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc1137296?pdf=render","host_type":"GREEN"},{"url":"https://ir.ymlib.yonsei.ac.kr/bitstream/22282913/195226/1/T199401692.pdf","host_type":"repository"},{"url":"https://portlandpress.com/biochemj/article-pdf/302/3/759/615252/bj3020759.pdf","host_type":"publisher"},{"url":"https://ir.ymlib.yonsei.ac.kr/handle/22282913/195226","host_type":"repository"},{"url":"https://doi.org/10.1042/bj3020759","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7945200","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1137296","host_type":"repository"}],"fields_of_study":["Drug Transport and Resistance Mechanisms","RNA modifications and cancer","RNA Interference and Gene Delivery","Biology","Medicine"],"mesh_terms":["Animals","ATP Citrate (pro-S)-Lyase","Base Sequence","Electrophoresis, Agar Gel","Molecular Sequence Data","Nucleic Acid Hybridization","Plasmids","Promoter Regions, Genetic","Regulatory Sequences, Nucleic Acid","Transcription, Genetic","Transfection","Blotting, Southern","Polymerase Chain Reaction","Rats"],"keywords":["ATP citrate lyase","Molecular biology","Primer extension","Chloramphenicol acetyltransferase","Biology","TATA box","CAAT box","5' flanking region","Gene","Nucleic acid sequence","Genomic library","Promoter","Gene expression","Genetics","Nucleotide","Peptide sequence","Biochemistry","Enzyme","Citrate synthase"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-04T07:40:32.702396Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}