{"doi":"10.1042/bj2940381","title":"Lack of stereospecificity of suid pseudorabies virus thymidine kinase","abstract":"<jats:p>We have partially purified suid pseudorabies virus (PRV) thymidine kinase from infected thymidine kinase- mouse cells, and cytosolic swine thymidine kinase from lymphatic glands, and we have found that PRV thymidine kinase, unlike the host enzyme, shows no stereospecificity for D- and L-beta-nucleosides. In vitro, unnatural L-enantiomers, except L-deoxycytidine, function as specific inhibitors for the viral enzyme in the order: L-thymidine &amp;gt;&amp;gt; L-deoxyguanosine &amp;gt; L-deoxyuridine &amp;gt; L-deoxyadenosine. Contrary to human and swine thymidine kinases and like herpes simplex virus-1 and -2 thymidine kinases, PRV thymidine kinase phosphorylates both the natural (D-) and the unnatural (L-) thymidine enantiomers to their corresponding monophosphates with comparable efficiency. The kinetic parameters Vmax/Km for D- and L-thymidine are 3.7 and 2.3 respectively. Our results demonstrate that the lack of stereospecificity might be a common feature of the thymidine kinases that are encoded by human and animal herpes viruses. These observations could lead to the development of a novel class of antiviral drugs.</jats:p>","journal":"Biochemical Journal","year":1993,"id":15880,"datarank":0.48460488484554715,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"self_citation_contribution":0.38474240361923057,"citation_network_contribution":0.09986248122631658,"self_endowment_contribution":0.38474240361923057,"citer_contribution":0.09986248122631658,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":3,"citers_with_citation_signal":3,"citers_with_endowment":3,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":119402,"name":"A Verri","orcid":null,"position":1,"is_corresponding":false},{"id":119403,"name":"L Bonizzi","orcid":null,"position":2,"is_corresponding":false},{"id":119405,"name":"W Ponti","orcid":null,"position":3,"is_corresponding":false},{"id":119407,"name":"G Poli","orcid":null,"position":4,"is_corresponding":false},{"id":119408,"name":"A Garbesi","orcid":null,"position":5,"is_corresponding":false},{"id":119409,"name":"D Niccolai","orcid":null,"position":6,"is_corresponding":false},{"id":119410,"name":"S Spadari","orcid":null,"position":7,"is_corresponding":false},{"id":119412,"name":"F Focher","orcid":null,"position":8,"is_corresponding":false},{"id":119401,"name":"G Maga","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8396911","pmcid":"PMC1134465","openalex_id":"https://openalex.org/W2324224710","authors":[],"funders":[],"total_grants":0,"fwci":0.6363,"citation_percentile":0.69963934,"influential_citations":1,"citation_trend":[],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://portlandpress.com/biochemj/article-pdf/294/2/381/724290/bj2940381.pdf","host_type":"journal"},{"url":"https://europepmc.org/articles/pmc1134465?pdf=render","host_type":"GREEN"},{"url":"https://portlandpress.com/biochemj/article-pdf/294/2/381/724290/bj2940381.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1042/bj2940381","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8396911","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1134465","host_type":"repository"}],"fields_of_study":["Herpesvirus Infections and Treatments","Virus-based gene therapy research","Poxvirus research and outbreaks","Biology","Medicine","Chemistry","Binding, Competitive","Deoxyadenosines","Deoxyguanosine","Deoxyuridine","Herpesvirus 1, Suid","Nucleosides","Phosphorylation","Stereoisomerism","Substrate Specificity","Thymidine","Thymidine Kinase"],"mesh_terms":["Binding, Competitive","Deoxyadenosines","Deoxyguanosine","Deoxyuridine","Nucleosides","Phosphorylation","Herpesvirus 1, Suid","Stereoisomerism","Substrate Specificity","Thymidine","Thymidine Kinase"],"keywords":["Pseudorabies","Thymidine kinase","Stereospecificity","Thymidine","Virology","Virus","Chemistry","Biology","Biochemistry","DNA","Herpes simplex virus"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-01T19:26:55.420981Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}