{"doi":"10.1042/bj2830371","title":"Characterization of phospholipase activity in <i>Dictyostelium discoideum</i>. Identification of a Ca2+-dependent polyphosphoinositide-specific phospholipase C","abstract":"<jats:p>We have identified a Ca(2+)-dependent polyphosphoinositide-specific phospholipase C activity in Dictyostelium discoideum. Addition of Ca2+ (20 microM) results in the rapid formation of Ins(1,4,5)P3 within 5 s and leads to sustained inositol phosphate production for up to 40 min in membranes prepared from [3H]inositol-labelled cells. The phospholipase C activity is primarily membrane-bound under the conditions used to lyse the cells. In addition to this activity we also identified a family of Ca(2+)-regulated phospholipase activities active on a range of phospholipid substrates, using [3H]palmitate labelling. Inositol-specific phospholipase C activity is highest in vegetatively growing cells and in starved cells during the first 6 h in development, during which time Ca2+ elicited a 5-fold stimulation of inositol phosphate formation. After this time, total activity decreased progressively until 15 h, after which the activity remained constant up until 24 h. During this period, Ca2+ was able to stimulate a 2-fold increase in inositol phosphates.</jats:p>","journal":"Biochemical Journal","year":1992,"id":684658,"datarank":0.42498200160843247,"base_score":2.833213344056216,"endowment":2.833213344056216,"self_citation_contribution":0.42498200160843247,"citation_network_contribution":0.0,"self_endowment_contribution":0.42498200160843247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1788665,"name":"R A Firtel","orcid":null,"position":1,"is_corresponding":false},{"id":1788664,"name":"A B Cubitt","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Characterization of phospholipase activity in <i>Dictyostelium discoideum</i>. Identification of a Ca2+-dependent polyphosphoinositide-specific phospholipase C","abstract":"<jats:p>We have identified a Ca(2+)-dependent polyphosphoinositide-specific phospholipase C activity in Dictyostelium discoideum. Addition of Ca2+ (20 microM) results in the rapid formation of Ins(1,4,5)P3 within 5 s and leads to sustained inositol phosphate production for up to 40 min in membranes prepared from [3H]inositol-labelled cells. The phospholipase C activity is primarily membrane-bound under the conditions used to lyse the cells. In addition to this activity we also identified a family of Ca(2+)-regulated phospholipase activities active on a range of phospholipid substrates, using [3H]palmitate labelling. Inositol-specific phospholipase C activity is highest in vegetatively growing cells and in starved cells during the first 6 h in development, during which time Ca2+ elicited a 5-fold stimulation of inositol phosphate formation. After this time, total activity decreased progressively until 15 h, after which the activity remained constant up until 24 h. During this period, Ca2+ was able to stimulate a 2-fold increase in inositol phosphates.</jats:p>","is_dataset_classified":null,"base_score":2.833213344056216,"endowment":2.833213344056216,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"1315514","pmcid":"PMC1131043","openalex_id":"https://openalex.org/W2239890886","authors":[],"funders":[],"total_grants":0,"fwci":1.2834,"citation_percentile":0.82230433,"influential_citations":0,"citation_trend":[],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://portlandpress.com/biochemj/article-pdf/283/2/371/606721/bj2830371.pdf","host_type":"journal"},{"url":"https://portlandpress.com/biochemj/article-pdf/283/2/371/606721/bj2830371.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1042/bj2830371","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/1315514","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1131043","host_type":"repository"}],"fields_of_study":["Cellular Mechanics and Interactions","Planarian Biology and Electrostimulation","3D Printing in Biomedical Research"],"mesh_terms":["Animals","Calcium","Cell Membrane","Chromatography, High Pressure Liquid","Dictyostelium","Inositol","Inositol Phosphates","Kinetics","Membrane Lipids","Palmitic Acids","Phosphoric Diester Hydrolases","Inositol 1,4,5-Trisphosphate","Guanosine 5'-O-(3-Thiotriphosphate)","Palmitic Acid","Phosphoinositide Phospholipase C"],"keywords":["Dictyostelium discoideum","Inositol","Phospholipase","Phospholipase C","Inositol phosphate","Phosphoinositide phospholipase C","Biochemistry","Phospholipid","Biology","Stimulation","Calcium","Specific activity","Enzyme","Membrane","Chemistry","Receptor","Endocrinology","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T14:41:12.161485Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}