{"doi":"10.1042/bj2410081","title":"Stimulation of choline release from NG108-15 cells by 12-<i>O</i>-tetradecanoylphorbol 13-acetate","abstract":"<jats:p>The effects of the potent tumour-promoting phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) on phosphatidylcholine (PtdCho) metabolism were investigated in the neuroblastoma X glioma hybrid cell line NG108-15. TPA (100 nM) stimulated by 150-200% the release into the medium of 3H radioactivity from cells that had been pre-labelled with [3H]choline. H.p.l.c. analysis of the medium revealed that TPA stimulated the release of only free [3H]choline (212 +/- 11% of control), without affecting such other labelled metabolites as [3H]phosphocholine and [3H]glycerophosphocholine. This effect was concentration-dependent, with a half-maximal effect obtained at 27.5 +/- 6.8 nM, and was observable as early as 5-10 min after exposure to TPA. The TPA-induced release of [3H]choline into the medium was accompanied by a small and variable decrease in cellular [3H]PtdCho (to 93 +/- 4% of control). However, the radioactivity associated with water-soluble cellular choline metabolites (mainly [3H]phosphocholine and [3H]glycerophosphocholine) remained unchanged. TPA also stimulated the release of [3H]choline derived from [3H]PtdCho that had been produced via the methylation pathway from [3H]methionine. These data suggest that phosphatidylcholine may serve as the source of free choline released from the cells in response to TPA. The possible enzymic mechanisms underlying this response are discussed.</jats:p>","journal":"Biochemical Journal","year":1987,"id":35399,"datarank":5.7473360737622725,"base_score":4.465908118654584,"endowment":4.465908118654584,"self_citation_contribution":0.6698862177981877,"citation_network_contribution":5.077449855964085,"self_endowment_contribution":0.6698862177981877,"citer_contribution":5.077449855964085,"corpus_percentile":null,"corpus_rank":null,"citation_count":86,"citer_count":75,"citers_with_citation_signal":73,"citers_with_endowment":73,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":181398,"name":"J K Blusztajn","orcid":null,"position":1,"is_corresponding":false},{"id":181399,"name":"A Freese","orcid":null,"position":2,"is_corresponding":false},{"id":181400,"name":"R J Wurtman","orcid":null,"position":3,"is_corresponding":false},{"id":181397,"name":"M Liscovitch","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":4.465908118654584,"endowment":4.465908118654584,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"3566713","pmcid":"PMC1147527","openalex_id":"https://openalex.org/W2259083375","authors":[],"funders":[],"total_grants":0,"fwci":7.6196,"citation_percentile":0.98100991,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2018,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://portlandpress.com/biochemj/article-pdf/241/1/81/588590/bj2410081.pdf","host_type":"journal"},{"url":"https://europepmc.org/articles/pmc1147527?pdf=render","host_type":"GREEN"},{"url":"https://portlandpress.com/biochemj/article-pdf/241/1/81/588590/bj2410081.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1042/bj2410081","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/3566713","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1147527","host_type":"repository"}],"fields_of_study":["Retinoids in leukemia and cellular processes","Cancer, Hypoxia, and Metabolism","Neurological Disorders and Treatments","Chemistry","Medicine","Biology","Cell Line","Choline","Chromatography, High Pressure Liquid","Ethanolamine","Ethanolamines","Methionine","Stimulation, Chemical","Tetradecanoylphorbol Acetate"],"mesh_terms":["Cell Line","Choline","Chromatography, High Pressure Liquid","Ethanolamines","Methionine","Stimulation, Chemical","Tetradecanoylphorbol Acetate","Ethanolamine"],"keywords":["Phosphocholine","Choline","Phosphatidylcholine","12-O-Tetradecanoylphorbol-13-acetate","Chemistry","Stimulation","Phospholipid","Choline kinase","Biochemistry","Metabolism","Methylation","Biology","Endocrinology","Membrane","Phosphorylation","Phorbol ester","Protein kinase C"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-10T03:46:12.548103Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}