{"doi":"10.1042/bj20100266","title":"Contribution of DEAH-box protein DHX16 in human pre-mRNA splicing","abstract":"<jats:p>Studies of mammalian splicing factors are often focused on small nuclear ribonucleoproteins or regulatory RNA-binding proteins, such as hnRNP (heterogeneous nuclear ribonucleoprotein) and SR proteins (serine/arginine-rich proteins); however, much less is known about the contribution of DExD/H-box proteins or RNA helicases in mammalian pre-mRNA splicing. The human DEAH-box protein DHX16 [also known as DBP2 (DEAD-box protein 2)], is homologous with Caenorhabditis elegans Mog-4, Schizosaccharomyces pombe Prp8 and Saccharomyces cerevisiae Prp2. In the present study, we show that DHX16 is required for pre-mRNA splicing after the formation of a pre-catalytic spliceosome. We found that anti-DHX16 antiserum inhibited the splicing reaction in vitro and the antibody immunoprecipitated pre-mRNA, splicing intermediates and spliceosomal small nuclear RNAs. Cells that expressed DHX16 that had a mutation in the helicase domain accumulated unspliced intron-containing minigene transcripts. Nuclear extracts isolated from the dominant-negative DHX16-G724N-expressing cells formed splicing complex B, but were impaired in splicing. Adding extracts containing DHX16-G724N or DHX16-S552L mutant proteins to HeLa cell nuclear extracts resulted in reduced splicing, indicating that the mutant protein directly inhibited splicing in vitro. Therefore our results show that DHX16 is needed for human pre-mRNA splicing at a step analogous to that mediated by the S. cerevisiae spliceosomal ATPase Prp2.</jats:p>","journal":"Biochemical Journal","year":2010,"id":34943,"datarank":1.593231154003847,"base_score":3.58351893845611,"endowment":3.58351893845611,"self_citation_contribution":0.5375278407684165,"citation_network_contribution":1.0557033132354305,"self_endowment_contribution":0.5375278407684165,"citer_contribution":1.0557033132354305,"corpus_percentile":null,"corpus_rank":null,"citation_count":35,"citer_count":31,"citers_with_citation_signal":26,"citers_with_endowment":26,"datacite_reuse_total":6,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":180050,"name":"Mitsuo Kato","orcid":null,"position":1,"is_corresponding":false},{"id":180051,"name":"Alain N.S. Newo","orcid":null,"position":2,"is_corresponding":false},{"id":180052,"name":"Ren-Jang Lin","orcid":null,"position":3,"is_corresponding":false},{"id":180049,"name":"Marieta Gencheva","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.58351893845611,"endowment":3.58351893845611,"datacite_reuse_total":6,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20423332","pmcid":"PMC3137565","openalex_id":"https://openalex.org/W1994196736","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM40639","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM040639","title":null}],"total_grants":2,"fwci":0.6805,"citation_percentile":0.68391402,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2014,"count":3},{"year":2017,"count":2},{"year":2018,"count":1},{"year":2019,"count":2},{"year":2020,"count":3},{"year":2021,"count":3},{"year":2022,"count":3},{"year":2023,"count":5},{"year":2024,"count":3},{"year":2025,"count":4},{"year":2026,"count":1}],"oa_status":"green","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3137565","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc3137565?pdf=render","host_type":"GREEN"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3137565","host_type":"repository"},{"url":"https://portlandpress.com/biochemj/article-pdf/429/1/25/662363/bj4290025.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1042/bj20100266","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/20423332","host_type":"repository"}],"fields_of_study":["RNA Research and Splicing","RNA modifications and cancer","RNA and protein synthesis mechanisms","Biology","Medicine","Catalysis","DEAD-box RNA Helicases","HeLa Cells","Humans","RNA Precursors","RNA Splicing","Saccharomyces cerevisiae Proteins","Spliceosomes"],"mesh_terms":["Catalysis","HeLa Cells","Humans","RNA Precursors","RNA Splicing","Spliceosomes","Saccharomyces cerevisiae Proteins","DEAD-box RNA Helicases","Hela Cells"],"keywords":["RNA splicing","Protein splicing","Spliceosome","Heterogeneous ribonucleoprotein particle","Minigene","Biology","Exonic splicing enhancer","SR protein","Small nuclear ribonucleoprotein","Intron","Ribonucleoprotein","RNA Helicase A","Cell biology","RNA-binding protein","Heterogeneous nuclear ribonucleoprotein","Splicing factor","Molecular biology","Polypyrimidine tract","RNA","Helicase","Genetics","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[{"doi":"10.6084/m9.figshare.16306023.v1","title":"Additional file 2 of Whole-genome methylation analysis of testicular germ cells from cryptozoospermic men points to recurrent and functionally relevant DNA methylation changes","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.16306023.v2","title":"Additional file 2 of Whole-genome methylation analysis of testicular germ cells from cryptozoospermic men points to recurrent and functionally relevant DNA methylation changes","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.16306023","title":"Additional file 2 of Whole-genome methylation analysis of testicular germ cells from cryptozoospermic men points to recurrent and functionally relevant DNA methylation changes","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.16306020.v1","title":"Additional file 1 of Whole-genome methylation analysis of testicular germ cells from cryptozoospermic men points to recurrent and functionally relevant DNA methylation changes","publisher":"figshare","resource_type":"Dataset"},{"doi":"10.6084/m9.figshare.16306020.v2","title":"Additional file 1 of Whole-genome methylation analysis of testicular germ cells from cryptozoospermic men points to recurrent and functionally relevant DNA methylation changes","publisher":"figshare","resource_type":"Dataset"},{"doi":"10.6084/m9.figshare.16306020","title":"Additional file 1 of Whole-genome methylation analysis of testicular germ cells from cryptozoospermic men points to recurrent and functionally relevant DNA methylation changes","publisher":"figshare","resource_type":"Dataset"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-09T22:10:08.346939Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}