{"doi":"10.1042/bj20020920","title":"Insulin stimulation of pyruvate dehydrogenase in adipocytes involves two distinct signalling pathways","abstract":"<jats:p>In isolated rat adipocytes, the insulin stimulation of pyruvate dehydrogenase can be partially inhibited by inhibitors of PI3K (phosphoinositide 3-kinase) and MEK1/2 (mitogen-activated protein kinase/extracellular signal-regulated kinase kinase). In combination, U0126 and wortmannin completely block the insulin stimulation of pyruvate dehydrogenase. It is concluded that the effect of insulin on pyruvate dehydrogenase in rat adipocytes involves two distinct signalling pathways: one is sensitive to wortmannin and the other to U0126. The synthetic phosphoinositolglycan PIG41 can activate pyruvate dehydrogenase but the activation is only approx. 30% of the maximal effect of insulin. This modest activation can be completely blocked by wortmannin alone, suggesting that PIG41 acts through only one of the pathways leading to the activation of pyruvate dehydrogenase.</jats:p>","journal":"Biochemical Journal","year":2003,"id":656360,"datarank":0.4493598410330987,"base_score":2.995732273553991,"endowment":2.995732273553991,"self_citation_contribution":0.4493598410330987,"citation_network_contribution":0.0,"self_endowment_contribution":0.4493598410330987,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1713386,"name":"Richard M. DENTON","orcid":null,"position":1,"is_corresponding":false},{"id":1713385,"name":"Sam A. JOHNSON","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Insulin stimulation of pyruvate dehydrogenase in adipocytes involves two distinct signalling pathways","abstract":"<jats:p>In isolated rat adipocytes, the insulin stimulation of pyruvate dehydrogenase can be partially inhibited by inhibitors of PI3K (phosphoinositide 3-kinase) and MEK1/2 (mitogen-activated protein kinase/extracellular signal-regulated kinase kinase). In combination, U0126 and wortmannin completely block the insulin stimulation of pyruvate dehydrogenase. It is concluded that the effect of insulin on pyruvate dehydrogenase in rat adipocytes involves two distinct signalling pathways: one is sensitive to wortmannin and the other to U0126. The synthetic phosphoinositolglycan PIG41 can activate pyruvate dehydrogenase but the activation is only approx. 30% of the maximal effect of insulin. This modest activation can be completely blocked by wortmannin alone, suggesting that PIG41 acts through only one of the pathways leading to the activation of pyruvate dehydrogenase.</jats:p>","is_dataset_classified":null,"base_score":2.995732273553991,"endowment":2.995732273553991,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"12374570","pmcid":"PMC1223089","openalex_id":"https://openalex.org/W2119507634","authors":[],"funders":[],"total_grants":0,"fwci":1.3804,"citation_percentile":0.79141566,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2020,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://portlandpress.com/biochemj/article-pdf/369/2/351/709524/bj3690351.pdf","host_type":"journal"},{"url":"https://portlandpress.com/biochemj/article-pdf/369/2/351/709524/bj3690351.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1042/bj20020920","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/12374570","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1223089","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.672.1337","host_type":""}],"fields_of_study":["Biochemical Acid Research Studies","Metabolism, Diabetes, and Cancer","Pancreatic function and diabetes","Adipocytes","Androstadienes","Animals","Butadienes","Cells, Cultured","Drug Synergism","Enzyme Activation","Enzyme Inhibitors","Insulin","MAP Kinase Kinase 1","Male","Mitogen-Activated Protein Kinase Kinases","Mitogen-Activated Protein Kinases","Nitriles","Phosphatidylinositol 3-Kinases","Phosphatidylinositols","Phosphoinositide-3 Kinase Inhibitors","Polysaccharides","Protein Serine-Threonine Kinases","Proto-Oncogene Proteins","Proto-Oncogene Proteins c-akt","Pyruvate Dehydrogenase Complex","Rats","Signal Transduction","Wortmannin"],"mesh_terms":["Wortmannin","Phosphoinositide-3 Kinase Inhibitors","Androstadienes","Animals","Butadienes","Cells, Cultured","Drug Synergism","Enzyme Activation","Enzyme Inhibitors","Insulin","Male","Nitriles","Phosphatidylinositols","Polysaccharides","Proto-Oncogene Proteins","Pyruvate Dehydrogenase Complex","Signal Transduction","Protein Serine-Threonine Kinases","Adipocytes","Phosphatidylinositol 3-Kinases","Mitogen-Activated Protein Kinases","Mitogen-Activated Protein Kinase Kinases","MAP Kinase Kinase 1","Proto-Oncogene Proteins c-akt","Rats"],"keywords":["Pyruvate dehydrogenase complex","Wortmannin","Pyruvate dehydrogenase kinase","Pyruvate dehydrogenase phosphatase","Pyruvate decarboxylation","Pyruvate dehydrogenase lipoamide kinase isozyme 1","Pyruvate kinase","PKM2","Chemistry","Oxoglutarate dehydrogenase complex","Biochemistry","Kinase","Biology","Cell biology","Glycolysis","Phosphatidylinositol","Enzyme"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-11T20:37:51.626258Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}