{"doi":"10.1042/bj1830065","title":"Inhibition of bacteriophage-Q β ribonucleic acid polymerase by guanosine triphosphate analogues","abstract":"<jats:p>Sixteen compounds related to GTP were evaluated as inhibitors of bacteriophage-Q beta poly(C)-dependent poly(G) polymerase. Non-phosphorylated compounds, including guanine, guanosine and deoxyguanosine, were inactive. Phosphorylated compounds gave significant inhibition at millimolar concentrations. For nucleotides the feature important for inhibition was the 5′-phosphate chain. Four triphosphates, XTP, ITP, 7-methyl-GTP and 2′-O-methyl-GTP, gave 50% inhibition of both the poly(C)- and poly(U2,C)-dependent reactions at concentrations from 0.1 to 5 mM. XTP was 10-fold more potent an inhibitor of the reaction with poly(U2,C) as template. None of these four compounds was able to substitute for GTP as substrate to a significant extent. The most active compound, 2′-O-methyl-GTP, was a competitive inhibitor (Ki = 0.4 mM) of GTP in the poly(C)-dependent reaction.</jats:p>","journal":"Biochemical Journal","year":1979,"id":43705,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":207084,"name":"R R Brooks","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"534489","pmcid":"PMC1161473","openalex_id":"https://openalex.org/W2402481472","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.23944781,"influential_citations":0,"citation_trend":[],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://portlandpress.com/biochemj/article-pdf/183/1/65/572574/bj1830065.pdf","host_type":"journal"},{"url":"https://europepmc.org/articles/pmc1161473?pdf=render","host_type":"GREEN"},{"url":"https://portlandpress.com/biochemj/article-pdf/183/1/65/572574/bj1830065.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1042/bj1830065","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/534489","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1161473","host_type":"repository"}],"fields_of_study":["Bacteriophages and microbial interactions","DNA and Nucleic Acid Chemistry","RNA and protein synthesis mechanisms","Chemistry","Medicine","Biology","Coliphages","DNA-Directed RNA Polymerases","Guanosine Triphosphate","Kinetics","Structure-Activity Relationship","Substrate Specificity"],"mesh_terms":["Coliphages","Guanosine Triphosphate","Kinetics","DNA-Directed RNA Polymerases","Structure-Activity Relationship","Substrate Specificity"],"keywords":["GTP'","Guanosine","Guanine","Guanosine triphosphate","Guanosine diphosphate","Nucleotide","Chemistry","Biochemistry","Stereochemistry","Non-competitive inhibition","Deoxyguanosine","Substrate (aquarium)","Molecular biology","Enzyme","DNA","Biology"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-15T02:39:07.986905Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}