{"doi":"10.1042/bj1810585","title":"Studies on the high-mobility-group non-histone proteins from hen oviduct","abstract":"<jats:p>Nuclear high-mobility-group (HMG) proteins were isolated from hen oviduct. These were proteins HMG-1, −2, −3, −14 and −17, which are equivalent to the classification of calf thymus HMG proteins. Hen oviduct proteins HMG-1 and −2 were individually isolated by HCIO4.extraction and CM-Sephadex chromatographic separation. Their mol.wts. were determined as 28 000 and 27 000, respectively. The proteins have a high content of acidic and basic amino acids. The association of proteins HMG-1 and −2 with the genome of hen oviduct nuclei was probed by a limited digestion with nucleases. Hen oviduct nuclei were incubated with deoxyribonuclease I or micrococcal nuclease until 10% of the DNA was digested. The nuclear suspension was centrifuged and the contents of proteins HMG-1 and −2 in the supernatant and sediment fractions were analysed by polyacrylamide-gel electrophoresis. HMG proteins were found to be preferentially released by micrococcal-nuclease digestion rather than by deoxyribonuclease I.</jats:p>","journal":"Biochemical Journal","year":1979,"id":15381,"datarank":1.4380829480426423,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"self_citation_contribution":0.47032413238937254,"citation_network_contribution":0.9677588156532696,"self_endowment_contribution":0.47032413238937254,"citer_contribution":0.9677588156532696,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":19,"citers_with_citation_signal":19,"citers_with_endowment":19,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":117567,"name":"G K Andrews","orcid":null,"position":1,"is_corresponding":false},{"id":117568,"name":"C T Teng","orcid":null,"position":2,"is_corresponding":false},{"id":117566,"name":"C S Teng","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"518542","pmcid":"PMC1161198","openalex_id":"https://openalex.org/W170100856","authors":[],"funders":[],"total_grants":0,"fwci":2.1343,"citation_percentile":0.86183743,"influential_citations":1,"citation_trend":[],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://portlandpress.com/biochemj/article-pdf/181/3/585/572465/bj1810585.pdf","host_type":"journal"},{"url":"https://europepmc.org/articles/pmc1161198?pdf=render","host_type":"GREEN"},{"url":"https://portlandpress.com/biochemj/article-pdf/181/3/585/572465/bj1810585.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1042/bj1810585","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/518542","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1161198","host_type":"repository"}],"fields_of_study":["Protein Hydrolysis and Bioactive Peptides","Natural product bioactivities and synthesis","Plant biochemistry and biosynthesis","Medicine","Biology","Amino Acids","Animals","Cattle","Cell Nucleus","Chickens","Chromosomal Proteins, Non-Histone","Deoxyribonucleases","Electrophoresis, Polyacrylamide Gel","Female","Micrococcal Nuclease","Oviducts","Thymus Gland"],"mesh_terms":["Amino Acids","Animals","Cattle","Cell Nucleus","Chickens","Chromosomal Proteins, Non-Histone","Deoxyribonucleases","Electrophoresis, Polyacrylamide Gel","Female","Micrococcal Nuclease","Oviducts","Thymus Gland"],"keywords":["Oviduct","Micrococcal nuclease","Deoxyribonuclease","High-mobility group","Nuclease","Sephadex","Non-histone protein","Biology","Biochemistry","DNA","Polyacrylamide gel electrophoresis","Deoxyribonuclease I","Digestion (alchemy)","Chromatin","Gel electrophoresis","Molecular biology","Chromatography","Enzyme","Chemistry","Gene","Endocrinology","Nucleosome"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-01T17:29:04.643060Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}