{"doi":"10.1042/bj1730309","title":"Involvement of deoxyribonucleic acid polymerase β in nuclear deoxyribonucleic acid synthesis","abstract":"<jats:p>The effects on DNA synthesis in vitro in mouse L929-cell nuclei of differential extraction of DNA polymerases alpha and beta were studied. Removal of all measurable DNA polymerase alpha and 20% of DNA polymerase beta leads to a 40% fall in the replicative DNA synthesis. Removal of 70% of DNA polymerase beta inhibits replicative synthesis by 80%. In all cases the nuclear DNA synthesis is sensitive to N-ethylmaleimide and aCTP (arabinosylcytosine triphosphate), though less so than DNA polymerase alpha. Addition of deoxyribonuclease I to the nuclear incubation leads to synthesis of high-molecular-weight DNA in a repair reaction. This occurs equally in nuclei from non-growing or S-phase cells. The former nuclei lack DNA polymerase alpha and the reaction reflects the sensitivity of DNA polymerase beta to inhibiton by N-ethylmaleimide and aCTP.</jats:p>","journal":"Biochemical Journal","year":1978,"id":684836,"datarank":0.42498200160843247,"base_score":2.833213344056216,"endowment":2.833213344056216,"self_citation_contribution":0.42498200160843247,"citation_network_contribution":0.0,"self_endowment_contribution":0.42498200160843247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1789232,"name":"W M Wood","orcid":null,"position":1,"is_corresponding":false},{"id":1789233,"name":"E L McKay","orcid":null,"position":2,"is_corresponding":false},{"id":1789238,"name":"R L P Adams","orcid":null,"position":3,"is_corresponding":false},{"id":1789229,"name":"T R Butt","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Involvement of deoxyribonucleic acid polymerase β in nuclear deoxyribonucleic acid synthesis","abstract":"<jats:p>The effects on DNA synthesis in vitro in mouse L929-cell nuclei of differential extraction of DNA polymerases alpha and beta were studied. Removal of all measurable DNA polymerase alpha and 20% of DNA polymerase beta leads to a 40% fall in the replicative DNA synthesis. Removal of 70% of DNA polymerase beta inhibits replicative synthesis by 80%. In all cases the nuclear DNA synthesis is sensitive to N-ethylmaleimide and aCTP (arabinosylcytosine triphosphate), though less so than DNA polymerase alpha. Addition of deoxyribonuclease I to the nuclear incubation leads to synthesis of high-molecular-weight DNA in a repair reaction. This occurs equally in nuclei from non-growing or S-phase cells. The former nuclei lack DNA polymerase alpha and the reaction reflects the sensitivity of DNA polymerase beta to inhibiton by N-ethylmaleimide and aCTP.</jats:p>","is_dataset_classified":null,"base_score":2.833213344056216,"endowment":2.833213344056216,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"687372","pmcid":"PMC1185775","openalex_id":"https://openalex.org/W2207980461","authors":[],"funders":[],"total_grants":0,"fwci":1.7875,"citation_percentile":0.84862256,"influential_citations":0,"citation_trend":[{"year":2024,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://portlandpress.com/biochemj/article-pdf/173/1/309/625039/bj1730309.pdf","host_type":"journal"},{"url":"https://portlandpress.com/biochemj/article-pdf/173/1/309/625039/bj1730309.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1042/bj1730309","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/687372","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1185775","host_type":"repository"}],"fields_of_study":["DNA Repair Mechanisms","Carcinogens and Genotoxicity Assessment","DNA and Nucleic Acid Chemistry","Animals","Cell Line","Cell Nucleus","Centrifugation, Density Gradient","DNA","DNA Polymerase I","DNA Polymerase II","DNA Repair","DNA-Directed DNA Polymerase","Ethylmaleimide","Mice","Nucleic Acid Synthesis Inhibitors"],"mesh_terms":["Animals","Cell Line","Cell Nucleus","Centrifugation, Density Gradient","DNA","DNA Polymerase I","DNA Polymerase II","DNA-Directed DNA Polymerase","DNA Repair","Ethylmaleimide","Nucleic Acid Synthesis Inhibitors","Mice"],"keywords":["DNA polymerase","DNA polymerase II","DNA clamp","Molecular biology","DNA synthesis","Polymerase","Biology","Deoxyribonuclease","DNA","DNA replication","DNA polymerase I","DNA polymerase mu","Primase","DNA polymerase delta","Biochemistry","Polymerase chain reaction","Circular bacterial chromosome","Gene","Reverse transcriptase"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T15:08:30.540154Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}