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Moreover, ATR FTIR spectroscopy reveals potential-dependent disulfide formation, which hints at an electron confurcation step between the metal centers. MicroScale thermophoresis indicates preferable binding between the HypCD complex and its in vivo interaction partner HypE under reducing conditions. Together, these results provide comprehensive evidence for an electron inventory fit to drive multi-electron redox reactions required for the assembly of the CN− and CO ligands on the scaffold complex HypCD.</jats:p>","journal":"Biochemical Journal","year":2021,"id":684196,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":1,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1419516,"name":"Jonathan Oltmanns","orcid":null,"position":1,"is_corresponding":false},{"id":1787429,"name":"Christina S. 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The data indicate a potential-dependent interconversion of the [Fe]2+/3+ and [4Fe-4S]2+/+ couple, respectively. Moreover, ATR FTIR spectroscopy reveals potential-dependent disulfide formation, which hints at an electron confurcation step between the metal centers. MicroScale thermophoresis indicates preferable binding between the HypCD complex and its in vivo interaction partner HypE under reducing conditions. Together, these results provide comprehensive evidence for an electron inventory fit to drive multi-electron redox reactions required for the assembly of the CN− and CO ligands on the scaffold complex HypCD.</jats:p>","is_dataset_classified":null,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"datacite_reuse_total":1,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34409988","pmcid":"PMC8454700","openalex_id":"https://openalex.org/W3195761187","authors":[],"funders":[{"funder_name":"Deutsche Forschungsgemeinschaft","grant_id":"273919336/SPP 1927","title":"Iron-Sulfur for Life"}],"total_grants":1,"fwci":0.3574,"citation_percentile":0.4768407,"influential_citations":0,"citation_trend":[{"year":2022,"count":4},{"year":2023,"count":3},{"year":2024,"count":1}],"oa_status":"hybrid","license":"cc-by-nc-nd","oa_locations":[{"url":"https://portlandpress.com/biochemj/article-pdf/478/17/3281/920249/bcj-2021-0224.pdf","host_type":"journal"},{"url":"https://portlandpress.com/biochemj/article-pdf/478/17/3281/920249/bcj-2021-0224.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1042/bcj20210224","host_type":"journal"},{"url":"http://europepmc.org/pmc/articles/PMC8454700","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8454700","host_type":"repository"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34409988","host_type":"repository"},{"url":"https://refubium.fu-berlin.de/handle/fub188/32826","host_type":"repository"},{"url":"https://doi.org/10.17169/refubium-32552","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC8454700","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC8454700?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.26434/chemrxiv.13736632.v1","host_type":""},{"url":"https://europepmc.org/articles/pmc8454700?pdf=render","host_type":""},{"url":"https://dx.doi.org/10.17169/refubium-32552","host_type":""},{"url":"http://dx.doi.org/10.1042/BCJ20210224","host_type":""},{"url":"https://dx.doi.org/10.1042/bcj20210224","host_type":""},{"url":"https://dx.doi.org/10.26434/chemrxiv.13736632.v1","host_type":""},{"url":"https://doi.org/https://doi.org/10.26434/chemrxiv.13736632.v1","host_type":""}],"fields_of_study":["Metalloenzymes and iron-sulfur proteins","Electrocatalysts for Energy Conversion","Advanced battery technologies research","0301 basic medicine","03 medical and health sciences"],"mesh_terms":["Carbon Monoxide","Disulfides","Electron Spin Resonance Spectroscopy","Electrons","Escherichia coli","Hydrogenase","Ions","Iron","Iron-Sulfur Proteins","Ligands","Oxidation-Reduction","Protein Binding","Proteins","Sulfur","Spectroscopy, Mossbauer","Spectroscopy, Fourier Transform Infrared","Catalytic Domain","Escherichia coli Proteins"],"keywords":["Cofactor","Hydrogenase","Iron–sulfur cluster","Electron paramagnetic resonance","Chemistry","Redox","Microscale thermophoresis","Electron transfer","Cluster (spacecraft)","Metal","Electron transport chain","Crystallography","Sulfur metabolism","Ligand (biochemistry)","Sulfur","Combinatorial chemistry","Stereochemistry","Inorganic chemistry","Photochemistry","Catalysis","Enzyme","Organic chemistry","Nuclear magnetic resonance","Biochemistry","Biosynthesis","Maturation","Carbon monoxide","Cyanide","Redox Activity","Fe-s Proteins","Iron-Sulfur Proteins","spectroscopy","Iron","Biophysics","Electrons","Ligands","bioinorganic chemistry","Spectroscopy, Mossbauer","redox chemistry","Catalytic Domain","Chemical Biology","Spectroscopy, Fourier Transform Infrared","Escherichia coli","Disulfides","Ions","Escherichia coli Proteins","Electron Spin Resonance Spectroscopy","Proteins","500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie","iron-sulfur clusters","Oxidation-Reduction","Protein Binding"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Clean water and sanitation"}],"linked_datasets":[{"doi":"10.17169/refubium-32552","title":"Electron inventory of the iron-sulfur scaffold complex HypCD essential in [NiFe]-hydrogenase cofactor assembly","publisher":"Freie Universität Berlin","resource_type":"Text"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T13:30:23.180143Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}