{"doi":"10.1039/d5sm00681c","title":"Loading causes molecular damage in fibrin fibers","abstract":". Indeed, many biological materials show distinct mechanical responses under repeated loading/unloading (cyclic loading), a prime example of which is clot embolization. Using lateral force microscopy, we show that fibrin fibers exhibit viscoelasticity and undergo irreversible molecular damage under cyclic loading. Cross-linking results in a more rigid structure with permanent damage occurring at larger strains - findings corroborated by computational modeling. Molecular spectroscopy analysis with broadband Raman scattering spectroscopy, combined with molecular dynamics simulations, allows identification of the damage source, unfolding pattern, and inter- and intramolecular changes in fibrin. The results reveal partial recovery of the protein's secondary and tertiary structures, providing a deeper understanding of fibrin's molecular wear under load and its behavior in wound healing and pathologies like stroke and embolism.","journal":"Soft Matter","year":2025,"id":542468,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9468,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1431512,"name":"Matthew J. Lohr","orcid":"0000-0003-3944-6651","position":1,"is_corresponding":false},{"id":1432042,"name":"Mayar T. Ibrahim","orcid":null,"position":2,"is_corresponding":false},{"id":781615,"name":"Christian M. Jennings","orcid":"0000-0003-4966-1582","position":3,"is_corresponding":false},{"id":241170,"name":"Daniel Wang","orcid":"0000-0003-4825-4547","position":4,"is_corresponding":false},{"id":381570,"name":"Pengyu Ren","orcid":"0000-0002-5613-1910","position":5,"is_corresponding":false},{"id":482423,"name":"Manuel K. Rausch","orcid":"0000-0003-1337-6472","position":6,"is_corresponding":false},{"id":140050,"name":"Sapun H. Parekh","orcid":"0000-0001-8522-1854","position":7,"is_corresponding":false},{"id":1103714,"name":"Sajjad Norouzi","orcid":null,"position":0,"is_corresponding":true}],"reference_count":66,"raw_metadata":null,"created_at":"2026-07-19T02:52:55.809501Z","pmid":"41122015","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}