{"doi":"10.1039/d5sm00659g","title":"Elasticity promotes directional transport of <i>Pseudomonas aeruginosa</i> in native human airway mucus and complex fluids","abstract":"strain PA14, a human clinical isolate responsible for chronic lung infections, in normal and diseased native human airway mucus. We use well-differentiated human bronchial epithelial cells cultured at the air-liquid-interface to secrete and harvest native human airway mucus with concentrations matching health and disease states. Furthermore, we develop a droplet-in-oil system for quantifying the transport of individual bacterium within bulk mucus. Remarkably, highly viscoelastic normal mucus promotes directional bacterial motility at a speed comparable to that in a low-viscosity physiological buffer. By contrast, concentrated, pathological mucus with elasticity dominating viscosity traps bacteria, reducing their motility more than 10-fold. Engineering mucus simulants with decoupled viscosity and elasticity reveals that the elasticity of complex fluids induces a qualitative change of bacterial motility from circular to directional motion. Our discovery not only provides insights into the biophysical mechanisms of bacterial infection in the lung but also reveals the previously unrecognized importance of elasticity in directional bacterial transport within complex fluids.","journal":"Soft Matter","year":2025,"id":579588,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9599,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":536014,"name":"Zhijian He","orcid":null,"position":1,"is_corresponding":false},{"id":356793,"name":"Li‐Heng Cai","orcid":"0000-0002-6806-0566","position":2,"is_corresponding":false},{"id":1490513,"name":"R. C. Dickson","orcid":null,"position":0,"is_corresponding":true}],"reference_count":78,"raw_metadata":null,"created_at":"2026-07-19T02:58:34.718602Z","pmid":"41395783","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}