{"doi":"10.1039/d5sc06380a","title":"Protein chirality as a determinant of ligand affinity: insights from <scp>l</scp> - and <scp>d</scp> -streptavidin","abstract":"mismatched biotin enantiomers that renders these systems functionally orthogonal. To gain further insight into how (-)-biotin binds recombinant streptavidin, we solved high-resolution X-ray crystal structures for both the matched and mismatched interactions. This work demonstrates the high degree of stereospecificity of the streptavidin/biotin interaction and the potential utility of a mirror-image biotin/streptavidin system for therapeutic and diagnostic applications.","journal":"Chemical Science","year":2025,"id":535207,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9487,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1419308,"name":"Peter C. S. Woodham","orcid":null,"position":1,"is_corresponding":false},{"id":1418780,"name":"Steven R. E. Draper","orcid":"0000-0001-7720-0340","position":2,"is_corresponding":false},{"id":802111,"name":"Paul Spaltenstein","orcid":"0000-0003-0476-7287","position":3,"is_corresponding":false},{"id":578665,"name":"Frank G. Whitby","orcid":"0000-0003-3511-2216","position":4,"is_corresponding":false},{"id":666493,"name":"Christopher P. Hill","orcid":"0000-0001-6796-7740","position":5,"is_corresponding":false},{"id":574402,"name":"Michael S. Kay","orcid":"0000-0003-3186-9684","position":6,"is_corresponding":false},{"id":1261335,"name":"Riley J. Giesler","orcid":"0000-0001-5786-0448","position":0,"is_corresponding":true}],"reference_count":66,"raw_metadata":null,"created_at":"2026-07-19T02:51:56.297114Z","pmid":"41210276","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}