{"doi":"10.1039/d4na00823e","title":"Binding affinity and transport studies of engineered photocrosslinkable affibody-enzyme-nanoparticle constructs","abstract":"values. However, the 17 nm particles conjugated with the photocrosslinkable N23BP affibody-enzymes showed an altered distribution profile that peaked further into the spheroid than the WT nanoparticle conjugates or in the absence of UV treatment. We correlate this finding with increased transport and retention of the photocrosslinked N23BP-nanoparticle conjugates in 3D spheroids to both the lower binding affinity of the affibodies for EGFR and the ability to introduce covalent linkages between the affibody and cell receptor. The larger 40 nm protein-conjugated nanoparticles showed limited penetration regardless of affinity or photocrosslinking on a 12 h timescale but did show overall increased transport after 24 h.","journal":"Nanoscale Advances","year":2025,"id":520630,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9483,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1284473,"name":"Bryce M. Bower","orcid":null,"position":1,"is_corresponding":false},{"id":1284472,"name":"Sven A. Saemundsson","orcid":null,"position":2,"is_corresponding":false},{"id":402814,"name":"Andrew P. Goodwin","orcid":"0000-0002-7284-4005","position":3,"is_corresponding":false},{"id":431939,"name":"N. Jennifer","orcid":"0000-0002-2840-1653","position":4,"is_corresponding":false},{"id":794457,"name":"Shane D. Curry","orcid":null,"position":0,"is_corresponding":true}],"reference_count":30,"raw_metadata":null,"created_at":"2026-07-19T02:49:28.470782Z","pmid":"40028492","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}