{"doi":"10.1039/d4cb00138a","title":"Early-stage biosynthesis of phenalinolactone diterpenoids involves sequential prenylation, epoxidation, and cyclization","abstract":"-perhydrophenanthrene core is constructed by sequential prenylation, epoxidation, and cyclization. The functions and timing of PlaT1-PlaT3 were assigned by comprehensive heterologous reconstitution. We illustrated that the UbiA prenyltransferase PlaT3 acts on geranylgeranyl diphosphate (GGPP) in the first step of phenalinolactone biosynthesis, prior to epoxidation by the flavin-dependent monooxygenase PlaT1 and cyclization by the type II terpene cyclase PlaT2. Finally, we isolated eight new-to-nature terpenoids, expanding the scope of the bacterial terpenome. The biosynthetic strategy employed in the assembly of the phenalinolactone core, with cyclization occurring after prenylation, is rare in bacteria and resembles fungal meroterpenoid biosynthesis. The findings presented here set the stage for future discovery, engineering, and enzymology efforts in bacterial meroterpenoids.","journal":"RSC Chemical Biology","year":2024,"id":447230,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9606,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":711099,"name":"Zining Li","orcid":"0000-0002-6921-8417","position":1,"is_corresponding":false},{"id":618634,"name":"Caitlin A. McCadden","orcid":"0009-0003-5165-4039","position":2,"is_corresponding":false},{"id":553977,"name":"Annika Jagels","orcid":"0000-0002-8461-2527","position":3,"is_corresponding":false},{"id":1265327,"name":"Diana P. Łomowska-Keehner","orcid":null,"position":4,"is_corresponding":false},{"id":1264843,"name":"Erin M Marshall","orcid":"0009-0000-0681-0600","position":5,"is_corresponding":false},{"id":379256,"name":"Liao‐Bin Dong","orcid":"0000-0002-2943-1299","position":6,"is_corresponding":false},{"id":588267,"name":"Sandra Loesgen","orcid":"0000-0003-1090-564X","position":7,"is_corresponding":false},{"id":568959,"name":"Jeffrey D. Rudolf","orcid":"0000-0003-2718-9651","position":8,"is_corresponding":false},{"id":618630,"name":"Tyler A. Alsup","orcid":"0000-0001-5868-0254","position":0,"is_corresponding":true}],"reference_count":30,"raw_metadata":null,"created_at":"2026-07-19T02:01:59.101070Z","pmid":"39144403","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}