{"doi":"10.1039/d3sc02864j","title":"Masked cerulenin enables a dual-site selective protein crosslink","abstract":"Protein-reactive natural products such as the fungal metabolite cerulenin are recognized for their value as therapeutic candidates, due to their ability to selectively react with catalytic residues within a protein active site or a complex of protein domains. Here, we explore the development of fatty-acid and polyketide-synthase probes by synthetically modulating cerulenin's functional moieties. Using a mechanism-based approach, we reveal unique reactivity within cerulenin and adapt it for fluorescent labeling and crosslinking of fatty-acid and iterative type-I polyketide synthases. We also describe two new classes of silylcyanohydrin and silylhemiaminal masked crosslinking probes that serve as new tools for activity and structure studies of these biosynthetic pathways.","journal":"Chemical Science","year":2023,"id":375506,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9517,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":295589,"name":"Aochiu Chen","orcid":"0000-0002-9143-7392","position":1,"is_corresponding":false},{"id":673753,"name":"Jeffrey Chen","orcid":"0000-0001-7542-0905","position":2,"is_corresponding":false},{"id":1138511,"name":"Arman Sekhon","orcid":null,"position":3,"is_corresponding":false},{"id":1138101,"name":"Gordon V. Louie","orcid":"0000-0002-8043-4348","position":4,"is_corresponding":false},{"id":295591,"name":"Joseph P. Noel","orcid":"0000-0002-1277-0331","position":5,"is_corresponding":false},{"id":295590,"name":"James J. La Clair","orcid":"0000-0001-6500-4107","position":6,"is_corresponding":false},{"id":295592,"name":"Michael D. Burkart","orcid":"0000-0002-4472-2254","position":7,"is_corresponding":false},{"id":905227,"name":"Ziran Jiang","orcid":"0000-0002-0086-4947","position":0,"is_corresponding":true}],"reference_count":39,"raw_metadata":null,"created_at":"2026-07-19T01:16:23.381203Z","pmid":"37829009","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}