{"doi":"10.1039/d3cb00027c","title":"Conservation of the insert-2 motif confers Rev1 from different species with an ability to disrupt G-quadruplexes and stimulate translesion DNA synthesis","abstract":"(lRev1) with hRev1, including an insert-2 mutant form of hRev1 (E466A/Y470A or EY). We found that zRev1 retained all of the G4-selective prowess of the human enzyme, but there was a marked attenuation of G4 binding affinity for the EY hRev1 mutant and the two Rev1 proteins lacking insert-2 (yRev1 and lRev1). Perhaps most strikingly, we found that insert-2 was important for disruption of the G4 structure and optimal stimulation of processive DNA synthesis across the guanine-rich motif by DNA polymerase kappa (pol κ). Our findings have implications for how Rev1 might contribute to G4 replication in different species spanning the evolutionary tree - signaling the importance of selection for enzymes with robust G4-selective properties in organisms where these non-B DNA structures may fulfill taxa-specific physiological functions.","journal":"RSC Chemical Biology","year":2023,"id":368576,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9571,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1127408,"name":"Reham S. Sewilam","orcid":null,"position":1,"is_corresponding":false},{"id":1126917,"name":"Mason McCrury","orcid":"0000-0001-5127-2696","position":2,"is_corresponding":false},{"id":1127409,"name":"Jaycelyn S. Hall","orcid":null,"position":3,"is_corresponding":false},{"id":1127410,"name":"Ashtyn Bell","orcid":null,"position":4,"is_corresponding":false},{"id":1127411,"name":"Bethany C. Paxton","orcid":null,"position":5,"is_corresponding":false},{"id":1127412,"name":"Shreyam Tripathi","orcid":null,"position":6,"is_corresponding":false},{"id":746199,"name":"Julie Gunderson","orcid":"0000-0003-0219-4390","position":7,"is_corresponding":false},{"id":391330,"name":"Robert L. Eoff","orcid":"0000-0003-4776-8925","position":8,"is_corresponding":false},{"id":570241,"name":"Amit Ketkar","orcid":"0000-0002-6536-7415","position":0,"is_corresponding":true}],"reference_count":104,"raw_metadata":null,"created_at":"2026-07-19T01:15:24.517012Z","pmid":"37415867","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}