{"doi":"10.1039/d3bm00340j","title":"Differentiation state and culture conditions impact neural stem/progenitor cell-derived extracellular vesicle bioactivity","abstract":"lack of optimization. Here, we assessed whether oligodendrocyte precursor cells (OPCs) and immature oligodendrocytes (iOLs), which are further differentiated than NPSCs and which both give rise to mature myelinating oligodendrocytes, could yield EVs with neurotherapeutic properties comparable or superior to those from NPSCs. We additionally examined the effects of extracellular matrix (ECM) coating materials and the presence or absence of growth factors in cell culture on the ultimate properties of EVs. The data show that OPC EVs and iOL EVs performed similarly to NPSC EVs in cell proliferation and anti-inflammatory assays, but NPSC EVs performed better in a neurite outgrowth assay. Additionally, the presence of nerve growth factor (NGF) in culture was found to maximize NPSC EV bioactivity among the conditions tested. NPSC EVs produced under rationally-selected culture conditions (fibronectin + NGF) enhanced axonal regeneration and muscle reinnervation in a rat nerve crush injury model. These results highlight the need for standardization of culture conditions for neurotherapeutic NPSC EV production.","journal":"Biomaterials Science","year":2023,"id":356241,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9603,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1029724,"name":"Nicholas H. Pirolli","orcid":"0000-0002-4163-1955","position":1,"is_corresponding":false},{"id":38114,"name":"Daniel Levy","orcid":"0000-0003-1843-8724","position":2,"is_corresponding":false},{"id":1105386,"name":"Jeffrey Y. Tsao","orcid":null,"position":3,"is_corresponding":false},{"id":1105387,"name":"Nicholas Seecharan","orcid":null,"position":4,"is_corresponding":false},{"id":1104970,"name":"Zihui Wang","orcid":"0000-0003-1051-7209","position":5,"is_corresponding":false},{"id":701758,"name":"Xiang Xu","orcid":"0000-0002-8977-8438","position":6,"is_corresponding":false},{"id":285547,"name":"Xiaofeng Jia","orcid":"0000-0003-1445-8525","position":7,"is_corresponding":false},{"id":328545,"name":"Steven M. Jay","orcid":"0000-0002-3827-5988","position":8,"is_corresponding":false},{"id":633984,"name":"Dipankar Dutta","orcid":"0000-0001-9864-1452","position":0,"is_corresponding":true}],"reference_count":61,"raw_metadata":null,"created_at":"2026-07-19T01:13:25.429924Z","pmid":"37367824","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}